Regulation of lactate dehydrogenase in response to WSSV infection in the shrimp Litopenaeus vannamei

M. L.E. Hernández-Palomares, J. A. Godoy-Lugo, S. Gómez-Jiménez, L. A. Gámez-Alejo, R. M. Ortiz, J. F. Muñoz-Valle, A. B. Peregrino-Uriarte, G. Yepiz-Plascencia, J. A. Rosas-Rodríguez, J. G. Soñanez-Organis

Research output: Contribution to journalArticle

Abstract

Lactate dehydrogenase (LDH) is key for anaerobic glycolysis. LDH is induced by the hypoxia inducible factor −1 (HIF-1). HIF-1 induces genes involved in glucose metabolism and regulates cellular oxygen homeostasis. HIF-1 is formed by a regulatory α-subunit (HIF-1α) and a constitutive β-subunit (HIF-1β). The white spot syndrome virus (WSSV) induces anaerobic glycolysis in shrimp hemocytes, associated with lactate accumulation. Although infection and lactate production are associated, the LDH role in WSSV-infected shrimp has not been examined. In this work, the effects of HIF-1 silencing on the expression of two LDH subunits (LDHvan-1 and LDHvan-2) in shrimp infected with the WSSV were studied. HIF-1α transcripts increased in gills, hepatopancreas, and muscle after WSSV infection, while HIF-1β remained constitutively expressed. The expression for both LDH subunits increased in each tissue evaluated during the WSSV infection, translating into increased enzyme activity. Glucose concentration increased in each tissue evaluated, while lactate increased in gills and hepatopancreas, but not in muscle. Silencing of HIF-1α blocked the increase of LDH expression and enzyme activity, along with glucose (all tissues) and lactate (gills and hepatopancreas) concentrations produced by WSSV infection. These results demonstrate that HIF-1 up regulates the expression of LDH subunits during WSSV infection, and that this induction contributes to substrate metabolism in energetically active tissues of infected shrimp.
Original languageAmerican English
Pages (from-to)401-409
Number of pages9
JournalFish and Shellfish Immunology
Volume74
DOIs
StatePublished - 1 Mar 2018

Fingerprint

Hypoxia-Inducible Factor 1
White spot syndrome virus
Litopenaeus vannamei
hypoxia
lactate dehydrogenase
Viruses
L-Lactate Dehydrogenase
virus
shrimp
infection
lactates
Lactic Acid
hepatopancreas
Tissue
gills
glucose
Enzyme activity
glycolysis
Metabolism
Glucose

Keywords

  • Gene expression
  • Gene silencing
  • Hypoxia-inducible factor -1α
  • Lactate dehydrogenase
  • White spot syndrome virus

Cite this

Hernández-Palomares, M. L. E., Godoy-Lugo, J. A., Gómez-Jiménez, S., Gámez-Alejo, L. A., Ortiz, R. M., Muñoz-Valle, J. F., ... Soñanez-Organis, J. G. (2018). Regulation of lactate dehydrogenase in response to WSSV infection in the shrimp Litopenaeus vannamei. Fish and Shellfish Immunology, 74, 401-409. https://doi.org/10.1016/j.fsi.2018.01.011
Hernández-Palomares, M. L.E. ; Godoy-Lugo, J. A. ; Gómez-Jiménez, S. ; Gámez-Alejo, L. A. ; Ortiz, R. M. ; Muñoz-Valle, J. F. ; Peregrino-Uriarte, A. B. ; Yepiz-Plascencia, G. ; Rosas-Rodríguez, J. A. ; Soñanez-Organis, J. G. / Regulation of lactate dehydrogenase in response to WSSV infection in the shrimp Litopenaeus vannamei. In: Fish and Shellfish Immunology. 2018 ; Vol. 74. pp. 401-409.
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abstract = "Lactate dehydrogenase (LDH) is key for anaerobic glycolysis. LDH is induced by the hypoxia inducible factor −1 (HIF-1). HIF-1 induces genes involved in glucose metabolism and regulates cellular oxygen homeostasis. HIF-1 is formed by a regulatory α-subunit (HIF-1α) and a constitutive β-subunit (HIF-1β). The white spot syndrome virus (WSSV) induces anaerobic glycolysis in shrimp hemocytes, associated with lactate accumulation. Although infection and lactate production are associated, the LDH role in WSSV-infected shrimp has not been examined. In this work, the effects of HIF-1 silencing on the expression of two LDH subunits (LDHvan-1 and LDHvan-2) in shrimp infected with the WSSV were studied. HIF-1α transcripts increased in gills, hepatopancreas, and muscle after WSSV infection, while HIF-1β remained constitutively expressed. The expression for both LDH subunits increased in each tissue evaluated during the WSSV infection, translating into increased enzyme activity. Glucose concentration increased in each tissue evaluated, while lactate increased in gills and hepatopancreas, but not in muscle. Silencing of HIF-1α blocked the increase of LDH expression and enzyme activity, along with glucose (all tissues) and lactate (gills and hepatopancreas) concentrations produced by WSSV infection. These results demonstrate that HIF-1 up regulates the expression of LDH subunits during WSSV infection, and that this induction contributes to substrate metabolism in energetically active tissues of infected shrimp.",
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Hernández-Palomares, MLE, Godoy-Lugo, JA, Gómez-Jiménez, S, Gámez-Alejo, LA, Ortiz, RM, Muñoz-Valle, JF, Peregrino-Uriarte, AB, Yepiz-Plascencia, G, Rosas-Rodríguez, JA & Soñanez-Organis, JG 2018, 'Regulation of lactate dehydrogenase in response to WSSV infection in the shrimp Litopenaeus vannamei', Fish and Shellfish Immunology, vol. 74, pp. 401-409. https://doi.org/10.1016/j.fsi.2018.01.011

Regulation of lactate dehydrogenase in response to WSSV infection in the shrimp Litopenaeus vannamei. / Hernández-Palomares, M. L.E.; Godoy-Lugo, J. A.; Gómez-Jiménez, S.; Gámez-Alejo, L. A.; Ortiz, R. M.; Muñoz-Valle, J. F.; Peregrino-Uriarte, A. B.; Yepiz-Plascencia, G.; Rosas-Rodríguez, J. A.; Soñanez-Organis, J. G.

In: Fish and Shellfish Immunology, Vol. 74, 01.03.2018, p. 401-409.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Regulation of lactate dehydrogenase in response to WSSV infection in the shrimp Litopenaeus vannamei

AU - Hernández-Palomares, M. L.E.

AU - Godoy-Lugo, J. A.

AU - Gómez-Jiménez, S.

AU - Gámez-Alejo, L. A.

AU - Ortiz, R. M.

AU - Muñoz-Valle, J. F.

AU - Peregrino-Uriarte, A. B.

AU - Yepiz-Plascencia, G.

AU - Rosas-Rodríguez, J. A.

AU - Soñanez-Organis, J. G.

PY - 2018/3/1

Y1 - 2018/3/1

N2 - Lactate dehydrogenase (LDH) is key for anaerobic glycolysis. LDH is induced by the hypoxia inducible factor −1 (HIF-1). HIF-1 induces genes involved in glucose metabolism and regulates cellular oxygen homeostasis. HIF-1 is formed by a regulatory α-subunit (HIF-1α) and a constitutive β-subunit (HIF-1β). The white spot syndrome virus (WSSV) induces anaerobic glycolysis in shrimp hemocytes, associated with lactate accumulation. Although infection and lactate production are associated, the LDH role in WSSV-infected shrimp has not been examined. In this work, the effects of HIF-1 silencing on the expression of two LDH subunits (LDHvan-1 and LDHvan-2) in shrimp infected with the WSSV were studied. HIF-1α transcripts increased in gills, hepatopancreas, and muscle after WSSV infection, while HIF-1β remained constitutively expressed. The expression for both LDH subunits increased in each tissue evaluated during the WSSV infection, translating into increased enzyme activity. Glucose concentration increased in each tissue evaluated, while lactate increased in gills and hepatopancreas, but not in muscle. Silencing of HIF-1α blocked the increase of LDH expression and enzyme activity, along with glucose (all tissues) and lactate (gills and hepatopancreas) concentrations produced by WSSV infection. These results demonstrate that HIF-1 up regulates the expression of LDH subunits during WSSV infection, and that this induction contributes to substrate metabolism in energetically active tissues of infected shrimp.

AB - Lactate dehydrogenase (LDH) is key for anaerobic glycolysis. LDH is induced by the hypoxia inducible factor −1 (HIF-1). HIF-1 induces genes involved in glucose metabolism and regulates cellular oxygen homeostasis. HIF-1 is formed by a regulatory α-subunit (HIF-1α) and a constitutive β-subunit (HIF-1β). The white spot syndrome virus (WSSV) induces anaerobic glycolysis in shrimp hemocytes, associated with lactate accumulation. Although infection and lactate production are associated, the LDH role in WSSV-infected shrimp has not been examined. In this work, the effects of HIF-1 silencing on the expression of two LDH subunits (LDHvan-1 and LDHvan-2) in shrimp infected with the WSSV were studied. HIF-1α transcripts increased in gills, hepatopancreas, and muscle after WSSV infection, while HIF-1β remained constitutively expressed. The expression for both LDH subunits increased in each tissue evaluated during the WSSV infection, translating into increased enzyme activity. Glucose concentration increased in each tissue evaluated, while lactate increased in gills and hepatopancreas, but not in muscle. Silencing of HIF-1α blocked the increase of LDH expression and enzyme activity, along with glucose (all tissues) and lactate (gills and hepatopancreas) concentrations produced by WSSV infection. These results demonstrate that HIF-1 up regulates the expression of LDH subunits during WSSV infection, and that this induction contributes to substrate metabolism in energetically active tissues of infected shrimp.

KW - Gene expression

KW - Gene silencing

KW - Hypoxia-inducible factor -1α

KW - Lactate dehydrogenase

KW - White spot syndrome virus

UR - http://www.mendeley.com/research/regulation-lactate-dehydrogenase-response-wssv-infection-shrimp-litopenaeus-vannamei

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M3 - Article

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JO - Fish and Shellfish Immunology

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Hernández-Palomares MLE, Godoy-Lugo JA, Gómez-Jiménez S, Gámez-Alejo LA, Ortiz RM, Muñoz-Valle JF et al. Regulation of lactate dehydrogenase in response to WSSV infection in the shrimp Litopenaeus vannamei. Fish and Shellfish Immunology. 2018 Mar 1;74:401-409. https://doi.org/10.1016/j.fsi.2018.01.011