Seasonality modulates the cellular antioxidant activity and antiproliferative effect of sonoran desert propolis

Pablo Mendez-Pfeiffer; Efrain Alday; Ana Laura Carreño; Jorge Hernández-Tánori; Beatriz Montaño-Leyva; Jesús Ortega-García; Judith Valdez; Adriana Garibay-Escobar; Javier Hernandez; Dora Valencia; Carlos Velazquez

doi:10.3390/antiox9121294

Seasonality modulates the cellular antioxidant activity and antiproliferative effect of sonoran desert propolis

Pablo Mendez-Pfeiffer, Efrain Alday, Ana Laura Carreño, Jorge Hernández-Tánori, Beatriz Montaño-Leyva, Jesús Ortega-García, Judith Valdez, Adriana Garibay-Escobar, Javier Hernandez, Dora Valencia, Carlos Velazquez

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Abstract

The main chemical composition and pharmacological potential of propolis from arid and semi-arid regions of the Sonoran Desert have been previously reported. Caborca propolis (CP), from an arid zone of the Sonoran Desert, has shown a polyphenolic profile that suggests a mixed plant origin, presenting poplar-type markers, as well as a 6-methoxylated flavonoid, xanthomicrol, characteristic of Asteraceae plants. In addition, CP has shown significant antioxidant properties and antiproliferative activity on cancer cells. In this study, we analyzed the influence of collection time on the chemical constitution, antiproliferative activity and protective capacity of CP against reactive oxygen species (ROS), by using HPLC–UV–diode array detection (DAD) analysis, 3-(4,5-dimethylthiazol-2-yl)-2,5-Dimethyltetrazoliumbromide (MTT) and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assays, as well as cellular antioxidant activity (CAA) assay on murine B-cell lymphoma M12.C3.F6 cells. HPLC–UV–DAD analyses of seasonally collected CP (one-year period) revealed quantitative differences among the most abundant CP constituents: pinocembrin, galangin, chrysin and pinobanksin-3-O-acetate. Though all seasonal samples of CP induced an antiproliferative effect in M12.C3.F6 cells, CP from autumn showed the highest inhibitory activity (IC : 5.9 ± 0.6 µg/mL). The DPPH assay pointed out that CP collected in autumn presented the highest antioxidant potential (IC : 58.8 ± 6.7 µg/mL), followed by winter (65.7 ± 12.2 µg/mL) and spring (67.0 ± 7.5 µg/mL); meanwhile, the summer sample showed a lesser antioxidant capacity (IC : 98.7 ± 2.5 µg/mL). The CAA assay demonstrated that CP induced a significant protective effect against ROS production elicited by H O in M12.C3.F6 cells. Pretreatment of M12.C3.F6 cells with CP from spring and autumn (25 and 50 µg/mL for 1 h) showed the highest reduction in intracellular ROS induced by H O (1 and 5 mM). These results indicate that the antiproliferative effect and cellular antioxidant activity of CP are modulated by quantitative fluctuations in its polyphenolic profile due to its collection time. 50 50 50 2 2 2 2

Original language	English
Article number	1294
Pages (from-to)	1-15
Number of pages	15
Journal	Antioxidants
Volume	9
Issue number	12
DOIs	https://doi.org/10.3390/antiox9121294
State	Published - 1 Dec 2020

Bibliographical note

Funding Information:
Funding: This research was funded by “Programa para el Desarrollo Profesional Docente, para el Tipo Superior (PRODEP)” grant UNISON-PTC-207.

Funding Information:
This research was funded by ?Programa para el Desarrollo Profesional Docente, para el Tipo Superior (PRODEP)? grant UNISON-PTC-207.

Funding Information:
Acknowledgments: The authors want to acknowledge Carlos A. Ortega Valenzuela, a beekeeper, for his help during propolis sample recollection, as well as M.Sc. Lucila Rascón for her technical support with this research. P.MP. acknowledges CONACyT fellowship No 617231.

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

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Mendez-Pfeiffer, P., Alday, E., Carreño, A. L., Hernández-Tánori, J., Montaño-Leyva, B., Ortega-García, J., Valdez, J., Garibay-Escobar, A., Hernandez, J., Valencia, D., & Velazquez, C. (2020). Seasonality modulates the cellular antioxidant activity and antiproliferative effect of sonoran desert propolis. Antioxidants, 9(12), 1-15. Article 1294. https://doi.org/10.3390/antiox9121294

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abstract = "The main chemical composition and pharmacological potential of propolis from arid and semi-arid regions of the Sonoran Desert have been previously reported. Caborca propolis (CP), from an arid zone of the Sonoran Desert, has shown a polyphenolic profile that suggests a mixed plant origin, presenting poplar-type markers, as well as a 6-methoxylated flavonoid, xanthomicrol, characteristic of Asteraceae plants. In addition, CP has shown significant antioxidant properties and antiproliferative activity on cancer cells. In this study, we analyzed the influence of collection time on the chemical constitution, antiproliferative activity and protective capacity of CP against reactive oxygen species (ROS), by using HPLC–UV–diode array detection (DAD) analysis, 3-(4,5-dimethylthiazol-2-yl)-2,5-Dimethyltetrazoliumbromide (MTT) and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assays, as well as cellular antioxidant activity (CAA) assay on murine B-cell lymphoma M12.C3.F6 cells. HPLC–UV–DAD analyses of seasonally collected CP (one-year period) revealed quantitative differences among the most abundant CP constituents: pinocembrin, galangin, chrysin and pinobanksin-3-O-acetate. Though all seasonal samples of CP induced an antiproliferative effect in M12.C3.F6 cells, CP from autumn showed the highest inhibitory activity (IC : 5.9 ± 0.6 µg/mL). The DPPH assay pointed out that CP collected in autumn presented the highest antioxidant potential (IC : 58.8 ± 6.7 µg/mL), followed by winter (65.7 ± 12.2 µg/mL) and spring (67.0 ± 7.5 µg/mL); meanwhile, the summer sample showed a lesser antioxidant capacity (IC : 98.7 ± 2.5 µg/mL). The CAA assay demonstrated that CP induced a significant protective effect against ROS production elicited by H O in M12.C3.F6 cells. Pretreatment of M12.C3.F6 cells with CP from spring and autumn (25 and 50 µg/mL for 1 h) showed the highest reduction in intracellular ROS induced by H O (1 and 5 mM). These results indicate that the antiproliferative effect and cellular antioxidant activity of CP are modulated by quantitative fluctuations in its polyphenolic profile due to its collection time. 50 50 50 2 2 2 2",

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Mendez-Pfeiffer, P, Alday, E, Carreño, AL, Hernández-Tánori, J, Montaño-Leyva, B , Ortega-García, J, Valdez, J, Garibay-Escobar, A, Hernandez, J, Valencia, D & Velazquez, C 2020, 'Seasonality modulates the cellular antioxidant activity and antiproliferative effect of sonoran desert propolis', Antioxidants, vol. 9, no. 12, 1294, pp. 1-15. https://doi.org/10.3390/antiox9121294

TY - JOUR

T1 - Seasonality modulates the cellular antioxidant activity and antiproliferative effect of sonoran desert propolis

AU - Mendez-Pfeiffer, Pablo

AU - Alday, Efrain

AU - Carreño, Ana Laura

AU - Hernández-Tánori, Jorge

AU - Montaño-Leyva, Beatriz

AU - Ortega-García, Jesús

AU - Valdez, Judith

AU - Garibay-Escobar, Adriana

AU - Hernandez, Javier

AU - Valencia, Dora

AU - Velazquez, Carlos

PY - 2020/12/1

Y1 - 2020/12/1

N2 - The main chemical composition and pharmacological potential of propolis from arid and semi-arid regions of the Sonoran Desert have been previously reported. Caborca propolis (CP), from an arid zone of the Sonoran Desert, has shown a polyphenolic profile that suggests a mixed plant origin, presenting poplar-type markers, as well as a 6-methoxylated flavonoid, xanthomicrol, characteristic of Asteraceae plants. In addition, CP has shown significant antioxidant properties and antiproliferative activity on cancer cells. In this study, we analyzed the influence of collection time on the chemical constitution, antiproliferative activity and protective capacity of CP against reactive oxygen species (ROS), by using HPLC–UV–diode array detection (DAD) analysis, 3-(4,5-dimethylthiazol-2-yl)-2,5-Dimethyltetrazoliumbromide (MTT) and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assays, as well as cellular antioxidant activity (CAA) assay on murine B-cell lymphoma M12.C3.F6 cells. HPLC–UV–DAD analyses of seasonally collected CP (one-year period) revealed quantitative differences among the most abundant CP constituents: pinocembrin, galangin, chrysin and pinobanksin-3-O-acetate. Though all seasonal samples of CP induced an antiproliferative effect in M12.C3.F6 cells, CP from autumn showed the highest inhibitory activity (IC : 5.9 ± 0.6 µg/mL). The DPPH assay pointed out that CP collected in autumn presented the highest antioxidant potential (IC : 58.8 ± 6.7 µg/mL), followed by winter (65.7 ± 12.2 µg/mL) and spring (67.0 ± 7.5 µg/mL); meanwhile, the summer sample showed a lesser antioxidant capacity (IC : 98.7 ± 2.5 µg/mL). The CAA assay demonstrated that CP induced a significant protective effect against ROS production elicited by H O in M12.C3.F6 cells. Pretreatment of M12.C3.F6 cells with CP from spring and autumn (25 and 50 µg/mL for 1 h) showed the highest reduction in intracellular ROS induced by H O (1 and 5 mM). These results indicate that the antiproliferative effect and cellular antioxidant activity of CP are modulated by quantitative fluctuations in its polyphenolic profile due to its collection time. 50 50 50 2 2 2 2

AB - The main chemical composition and pharmacological potential of propolis from arid and semi-arid regions of the Sonoran Desert have been previously reported. Caborca propolis (CP), from an arid zone of the Sonoran Desert, has shown a polyphenolic profile that suggests a mixed plant origin, presenting poplar-type markers, as well as a 6-methoxylated flavonoid, xanthomicrol, characteristic of Asteraceae plants. In addition, CP has shown significant antioxidant properties and antiproliferative activity on cancer cells. In this study, we analyzed the influence of collection time on the chemical constitution, antiproliferative activity and protective capacity of CP against reactive oxygen species (ROS), by using HPLC–UV–diode array detection (DAD) analysis, 3-(4,5-dimethylthiazol-2-yl)-2,5-Dimethyltetrazoliumbromide (MTT) and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assays, as well as cellular antioxidant activity (CAA) assay on murine B-cell lymphoma M12.C3.F6 cells. HPLC–UV–DAD analyses of seasonally collected CP (one-year period) revealed quantitative differences among the most abundant CP constituents: pinocembrin, galangin, chrysin and pinobanksin-3-O-acetate. Though all seasonal samples of CP induced an antiproliferative effect in M12.C3.F6 cells, CP from autumn showed the highest inhibitory activity (IC : 5.9 ± 0.6 µg/mL). The DPPH assay pointed out that CP collected in autumn presented the highest antioxidant potential (IC : 58.8 ± 6.7 µg/mL), followed by winter (65.7 ± 12.2 µg/mL) and spring (67.0 ± 7.5 µg/mL); meanwhile, the summer sample showed a lesser antioxidant capacity (IC : 98.7 ± 2.5 µg/mL). The CAA assay demonstrated that CP induced a significant protective effect against ROS production elicited by H O in M12.C3.F6 cells. Pretreatment of M12.C3.F6 cells with CP from spring and autumn (25 and 50 µg/mL for 1 h) showed the highest reduction in intracellular ROS induced by H O (1 and 5 mM). These results indicate that the antiproliferative effect and cellular antioxidant activity of CP are modulated by quantitative fluctuations in its polyphenolic profile due to its collection time. 50 50 50 2 2 2 2

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DO - 10.3390/antiox9121294

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AN - SCOPUS:85098270830

SN - 2076-3921

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JO - Antioxidants

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ER -

Seasonality modulates the cellular antioxidant activity and antiproliferative effect of sonoran desert propolis

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