Silencing of HIF-1 in WSSV-infected white shrimp

Effect on viral load and antioxidant enzymes

Melissa M Miranda-Cruz, Jennifer J Poom-Llamas, José A Godoy-Lugo, Rudy M Ortiz, Silvia Gómez-Jiménez, Jesús A Rosas-Rodríguez, Edgar F Morán-Palacio, José G Soñanez-Organis

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Hypoxia inducible factor-1 (HIF-1) is a transcriptional factor that induces genes involved in glucose metabolism. HIF-1 is formed by a regulatory α-subunit (HIF-1α) and a constitutive β-subunit (HIF-1β). The white spot syndrome virus (WSSV) induces a shift in glucose metabolism and oxidative stress. HIF-1α is associated with the induction of metabolic changes in tissues of WSSV-infected shrimp. However, the contributions of HIF-1 to viral load and antioxidant responses in WSSV-infected shrimp have been not examined. In this study, the effect of HIF-1 silencing on viral load and the expression and activity of antioxidant enzymes (superoxide dismutase-SOD, glutathione S-transferase-GST, and catalase) along with oxidative damage (lipid peroxidation and protein carbonyl) in tissues of white shrimp infected with the WSSV were studied. The viral load increased in hepatopancreas and muscle after WSSV infection, and the accumulative mortality was of 100% at 72 h post-infection. The expression and activity of SOD, catalase, and GST decreased in each tissue evaluated after WSSV infection. Protein carbonyl concentrations increased in each tissue after WSSV infection, while lipid peroxidation increased in hepatopancreas, but not in muscle. Silencing of HIF-1α decreased the WSSV viral load in hepatopancreas and muscle of infected shrimp along with shrimp mortality. Silencing of HIF-1α ameliorated the antioxidant response in a tissue-specific manner, which translated to a decrease in oxidative damage. These results suggest that HIF-1 is essential for restoring the antioxidant response, which counters the oxidative injury associated with WSSV infection.

Original languageEnglish
Pages (from-to)19-26
Number of pages8
JournalComparative Biochemistry and Physiology - C Toxicology and Pharmacology
Volume213
DOIs
StatePublished - Nov 2018
Externally publishedYes

Keywords

  • Animals
  • Aquaculture
  • DNA, Viral/isolation & purification
  • Gene Expression Regulation, Developmental
  • Gene Silencing
  • Hepatopancreas/growth & development
  • Hypoxia-Inducible Factor 1, alpha Subunit/antagonists & inhibitors
  • Injections, Intramuscular
  • Lipid Peroxidation
  • Mexico
  • Muscles/metabolism
  • Organ Specificity
  • Oxidative Stress
  • Oxidoreductases/genetics
  • Penaeidae/growth & development
  • Protein Carbonylation
  • RNA Interference
  • RNA, Double-Stranded/administration & dosage
  • Viral Load
  • White spot syndrome virus 1/isolation & purification

Cite this

@article{8130083a087741ec87a78b3af23ab978,
title = "Silencing of HIF-1 in WSSV-infected white shrimp: Effect on viral load and antioxidant enzymes",
abstract = "Hypoxia inducible factor-1 (HIF-1) is a transcriptional factor that induces genes involved in glucose metabolism. HIF-1 is formed by a regulatory α-subunit (HIF-1α) and a constitutive β-subunit (HIF-1β). The white spot syndrome virus (WSSV) induces a shift in glucose metabolism and oxidative stress. HIF-1α is associated with the induction of metabolic changes in tissues of WSSV-infected shrimp. However, the contributions of HIF-1 to viral load and antioxidant responses in WSSV-infected shrimp have been not examined. In this study, the effect of HIF-1 silencing on viral load and the expression and activity of antioxidant enzymes (superoxide dismutase-SOD, glutathione S-transferase-GST, and catalase) along with oxidative damage (lipid peroxidation and protein carbonyl) in tissues of white shrimp infected with the WSSV were studied. The viral load increased in hepatopancreas and muscle after WSSV infection, and the accumulative mortality was of 100{\%} at 72 h post-infection. The expression and activity of SOD, catalase, and GST decreased in each tissue evaluated after WSSV infection. Protein carbonyl concentrations increased in each tissue after WSSV infection, while lipid peroxidation increased in hepatopancreas, but not in muscle. Silencing of HIF-1α decreased the WSSV viral load in hepatopancreas and muscle of infected shrimp along with shrimp mortality. Silencing of HIF-1α ameliorated the antioxidant response in a tissue-specific manner, which translated to a decrease in oxidative damage. These results suggest that HIF-1 is essential for restoring the antioxidant response, which counters the oxidative injury associated with WSSV infection.",
keywords = "Animals, Aquaculture, DNA, Viral/isolation & purification, Gene Expression Regulation, Developmental, Gene Silencing, Hepatopancreas/growth & development, Hypoxia-Inducible Factor 1, alpha Subunit/antagonists & inhibitors, Injections, Intramuscular, Lipid Peroxidation, Mexico, Muscles/metabolism, Organ Specificity, Oxidative Stress, Oxidoreductases/genetics, Penaeidae/growth & development, Protein Carbonylation, RNA Interference, RNA, Double-Stranded/administration & dosage, Viral Load, White spot syndrome virus 1/isolation & purification",
author = "Miranda-Cruz, {Melissa M} and Poom-Llamas, {Jennifer J} and Godoy-Lugo, {Jos{\'e} A} and Ortiz, {Rudy M} and Silvia G{\'o}mez-Jim{\'e}nez and Rosas-Rodr{\'i}guez, {Jes{\'u}s A} and Mor{\'a}n-Palacio, {Edgar F} and So{\~n}anez-Organis, {Jos{\'e} G}",
note = "Copyright {\circledC} 2018 Elsevier Inc. All rights reserved.",
year = "2018",
month = "11",
doi = "10.1016/j.cbpc.2018.07.004",
language = "Ingl{\'e}s",
volume = "213",
pages = "19--26",
journal = "Comparative Biochemistry and Physiology - C Toxicology and Pharmacology",
issn = "1532-0456",
publisher = "Elsevier Inc.",

}

Silencing of HIF-1 in WSSV-infected white shrimp : Effect on viral load and antioxidant enzymes. / Miranda-Cruz, Melissa M; Poom-Llamas, Jennifer J; Godoy-Lugo, José A; Ortiz, Rudy M; Gómez-Jiménez, Silvia; Rosas-Rodríguez, Jesús A; Morán-Palacio, Edgar F; Soñanez-Organis, José G.

In: Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, Vol. 213, 11.2018, p. 19-26.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Silencing of HIF-1 in WSSV-infected white shrimp

T2 - Effect on viral load and antioxidant enzymes

AU - Miranda-Cruz, Melissa M

AU - Poom-Llamas, Jennifer J

AU - Godoy-Lugo, José A

AU - Ortiz, Rudy M

AU - Gómez-Jiménez, Silvia

AU - Rosas-Rodríguez, Jesús A

AU - Morán-Palacio, Edgar F

AU - Soñanez-Organis, José G

N1 - Copyright © 2018 Elsevier Inc. All rights reserved.

PY - 2018/11

Y1 - 2018/11

N2 - Hypoxia inducible factor-1 (HIF-1) is a transcriptional factor that induces genes involved in glucose metabolism. HIF-1 is formed by a regulatory α-subunit (HIF-1α) and a constitutive β-subunit (HIF-1β). The white spot syndrome virus (WSSV) induces a shift in glucose metabolism and oxidative stress. HIF-1α is associated with the induction of metabolic changes in tissues of WSSV-infected shrimp. However, the contributions of HIF-1 to viral load and antioxidant responses in WSSV-infected shrimp have been not examined. In this study, the effect of HIF-1 silencing on viral load and the expression and activity of antioxidant enzymes (superoxide dismutase-SOD, glutathione S-transferase-GST, and catalase) along with oxidative damage (lipid peroxidation and protein carbonyl) in tissues of white shrimp infected with the WSSV were studied. The viral load increased in hepatopancreas and muscle after WSSV infection, and the accumulative mortality was of 100% at 72 h post-infection. The expression and activity of SOD, catalase, and GST decreased in each tissue evaluated after WSSV infection. Protein carbonyl concentrations increased in each tissue after WSSV infection, while lipid peroxidation increased in hepatopancreas, but not in muscle. Silencing of HIF-1α decreased the WSSV viral load in hepatopancreas and muscle of infected shrimp along with shrimp mortality. Silencing of HIF-1α ameliorated the antioxidant response in a tissue-specific manner, which translated to a decrease in oxidative damage. These results suggest that HIF-1 is essential for restoring the antioxidant response, which counters the oxidative injury associated with WSSV infection.

AB - Hypoxia inducible factor-1 (HIF-1) is a transcriptional factor that induces genes involved in glucose metabolism. HIF-1 is formed by a regulatory α-subunit (HIF-1α) and a constitutive β-subunit (HIF-1β). The white spot syndrome virus (WSSV) induces a shift in glucose metabolism and oxidative stress. HIF-1α is associated with the induction of metabolic changes in tissues of WSSV-infected shrimp. However, the contributions of HIF-1 to viral load and antioxidant responses in WSSV-infected shrimp have been not examined. In this study, the effect of HIF-1 silencing on viral load and the expression and activity of antioxidant enzymes (superoxide dismutase-SOD, glutathione S-transferase-GST, and catalase) along with oxidative damage (lipid peroxidation and protein carbonyl) in tissues of white shrimp infected with the WSSV were studied. The viral load increased in hepatopancreas and muscle after WSSV infection, and the accumulative mortality was of 100% at 72 h post-infection. The expression and activity of SOD, catalase, and GST decreased in each tissue evaluated after WSSV infection. Protein carbonyl concentrations increased in each tissue after WSSV infection, while lipid peroxidation increased in hepatopancreas, but not in muscle. Silencing of HIF-1α decreased the WSSV viral load in hepatopancreas and muscle of infected shrimp along with shrimp mortality. Silencing of HIF-1α ameliorated the antioxidant response in a tissue-specific manner, which translated to a decrease in oxidative damage. These results suggest that HIF-1 is essential for restoring the antioxidant response, which counters the oxidative injury associated with WSSV infection.

KW - Animals

KW - Aquaculture

KW - DNA, Viral/isolation & purification

KW - Gene Expression Regulation, Developmental

KW - Gene Silencing

KW - Hepatopancreas/growth & development

KW - Hypoxia-Inducible Factor 1, alpha Subunit/antagonists & inhibitors

KW - Injections, Intramuscular

KW - Lipid Peroxidation

KW - Mexico

KW - Muscles/metabolism

KW - Organ Specificity

KW - Oxidative Stress

KW - Oxidoreductases/genetics

KW - Penaeidae/growth & development

KW - Protein Carbonylation

KW - RNA Interference

KW - RNA, Double-Stranded/administration & dosage

KW - Viral Load

KW - White spot syndrome virus 1/isolation & purification

U2 - 10.1016/j.cbpc.2018.07.004

DO - 10.1016/j.cbpc.2018.07.004

M3 - Artículo

VL - 213

SP - 19

EP - 26

JO - Comparative Biochemistry and Physiology - C Toxicology and Pharmacology

JF - Comparative Biochemistry and Physiology - C Toxicology and Pharmacology

SN - 1532-0456

ER -