Synthesis of Gold Nanoparticles Using Mimosa tenuiflora Extract, Assessments of Cytotoxicity, Cellular Uptake, and Catalysis

Ericka Rodríguez-León, Blanca E. Rodríguez-Vázquez, Aarón Martínez-Higuera, César Rodríguez-Beas, Eduardo Larios-Rodríguez, Rosa E. Navarro, Ricardo López-Esparza, Ramón A. Iñiguez-Palomares*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

54 Scopus citations

Abstract

Synthesis of gold nanoparticles (AuNPs) with plant extracts has gained great interest in the field of biomedicine due to its wide variety of health applications. In the present work, AuNPs were synthesized with Mimosa tenuiflora (Mt) bark extract at different metallic precursor concentrations. Mt extract was obtained by mixing the tree bark in ethanol-water. The antioxidant capacity of extract was evaluated using 2,2-diphenyl-1-picrylhydrazyl and total polyphenol assay. AuNPs were characterized by transmission electron microscopy, X-ray diffraction, UV-Vis and Fourier transform infrared spectroscopy, and X-ray photoelectron spectrometry for functional group determination onto their surface. AuMt (colloids formed by AuNPs and molecules of Mt) exhibit multiple shapes with sizes between 20 and 200 nm. AuMt were tested on methylene blue degradation in homogeneous catalysis adding sodium borohydride. The smallest NPs (AuMt1) have a degradation coefficient of 0.008/s and reach 50% degradation in 190s. Cell viability and cytotoxicity were evaluated in human umbilical vein endothelial cells (HUVEC), and a moderate cytotoxic effect at 24 and 48 h was found. However, toxicity does not behave in a dose-dependent manner. Cellular internalization of AuMt on HUVEC cells was analyzed by confocal laser scanning microscopy. For AuMt1, it can be observed that the material is dispersed into the cytoplasm, while in AuMt2, the material is concentrated in the nuclear periphery.

Original languageEnglish
Article number334
JournalNanoscale Research Letters
Volume14
Issue number1
DOIs
StatePublished - 1 Jan 2019
Externally publishedYes

Bibliographical note

Funding Information:
Authors would like to thank Laboratorio de Microscopía Electrónica de Transmisión and Laboratorio de Biomateriales of the University of Sonora.

Publisher Copyright:
© 2019, The Author(s).

Keywords

  • Catalysis
  • Cellular uptake
  • Gold
  • Nanoparticles

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