Taxonomic and functional changes in the microbiota of the white shrimp (Litopenaeus vannamei) associated with postlarval ontogenetic development

Estefanía Garibay-Valdez, Marcel Martínez-Porchas, Kadiya Calderon, Francisco Vargas-Albores, Teresa Gollas-Galván, Luis Rafael Martínez Córdova

Research output: Contribution to journalArticle

Abstract

Even though Litopenaeus vannamei is one of the most important species used in aquaculture, little is known about the functions provided by the intestinal microbiota for host development. This study aims to determine the taxonomic and functional changes in the L. vannamei gut microbiota during postlarval development in a recirculation system under controlled conditions for 80 days. The results revealed that the Vibrionaceae family predominated at the beginning of the experiment, when the postlarvae weighed 0.5 to 1.5 g. The representativeness of the family Rhodobacteraceae increased to the detriment of Vibrionaceae, which showed a constant decrease as the shrimp reached the juvenile and adult stages. Finally, the Intrasporangiaceae family remained constant in postlarvae weighing 4 to 10 g. Differences in the taxonomic profile at the family level between culture days were observed through principal component analysis (PCA), where two main clusters were observed: a group of microbiota sampled at 0 D and 20 D and another of samples collected at 40 D, 60 D and 80 D, suggesting that the microbiota tend to be variable during the first postlarval phase but become more constant towards the adult phase. Microbial-mediated functions predicted by PICRUSt showed an overall functional redundancy, suggesting that gut conditions maintain the same microbiota functions regardless of changes in the taxonomic structure. These results also suggest that shrimp are under certain selective pressure favoring microbiota with specific functions according to their requirements.
Original languageAmerican English
Pages (from-to)734842
Number of pages734842
JournalAquaculture
DOIs
StatePublished - 2020

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functional change
Litopenaeus vannamei
shrimp
Vibrionaceae
postlarvae
intestinal microorganisms
Intrasporangiaceae
Rhodobacteraceae
recirculating aquaculture systems
aquaculture
principal component analysis
digestive system
microbiome
family
experiment
sampling

Cite this

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title = "Taxonomic and functional changes in the microbiota of the white shrimp (Litopenaeus vannamei) associated with postlarval ontogenetic development",
abstract = "Even though Litopenaeus vannamei is one of the most important species used in aquaculture, little is known about the functions provided by the intestinal microbiota for host development. This study aims to determine the taxonomic and functional changes in the L. vannamei gut microbiota during postlarval development in a recirculation system under controlled conditions for 80 days. The results revealed that the Vibrionaceae family predominated at the beginning of the experiment, when the postlarvae weighed 0.5 to 1.5 g. The representativeness of the family Rhodobacteraceae increased to the detriment of Vibrionaceae, which showed a constant decrease as the shrimp reached the juvenile and adult stages. Finally, the Intrasporangiaceae family remained constant in postlarvae weighing 4 to 10 g. Differences in the taxonomic profile at the family level between culture days were observed through principal component analysis (PCA), where two main clusters were observed: a group of microbiota sampled at 0 D and 20 D and another of samples collected at 40 D, 60 D and 80 D, suggesting that the microbiota tend to be variable during the first postlarval phase but become more constant towards the adult phase. Microbial-mediated functions predicted by PICRUSt showed an overall functional redundancy, suggesting that gut conditions maintain the same microbiota functions regardless of changes in the taxonomic structure. These results also suggest that shrimp are under certain selective pressure favoring microbiota with specific functions according to their requirements.",
author = "Estefan{\'i}a Garibay-Valdez and Marcel Mart{\'i}nez-Porchas and Kadiya Calderon and Francisco Vargas-Albores and Teresa Gollas-Galv{\'a}n and {Mart{\'i}nez C{\'o}rdova}, {Luis Rafael}",
year = "2020",
doi = "10.1016/j.aquaculture.2019.734842",
language = "American English",
pages = "734842",
journal = "Aquaculture",
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Taxonomic and functional changes in the microbiota of the white shrimp (Litopenaeus vannamei) associated with postlarval ontogenetic development. / Garibay-Valdez, Estefanía; Martínez-Porchas, Marcel; Calderon, Kadiya; Vargas-Albores, Francisco; Gollas-Galván, Teresa; Martínez Córdova, Luis Rafael.

In: Aquaculture, 2020, p. 734842.

Research output: Contribution to journalArticle

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T1 - Taxonomic and functional changes in the microbiota of the white shrimp (Litopenaeus vannamei) associated with postlarval ontogenetic development

AU - Garibay-Valdez, Estefanía

AU - Martínez-Porchas, Marcel

AU - Calderon, Kadiya

AU - Vargas-Albores, Francisco

AU - Gollas-Galván, Teresa

AU - Martínez Córdova, Luis Rafael

PY - 2020

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N2 - Even though Litopenaeus vannamei is one of the most important species used in aquaculture, little is known about the functions provided by the intestinal microbiota for host development. This study aims to determine the taxonomic and functional changes in the L. vannamei gut microbiota during postlarval development in a recirculation system under controlled conditions for 80 days. The results revealed that the Vibrionaceae family predominated at the beginning of the experiment, when the postlarvae weighed 0.5 to 1.5 g. The representativeness of the family Rhodobacteraceae increased to the detriment of Vibrionaceae, which showed a constant decrease as the shrimp reached the juvenile and adult stages. Finally, the Intrasporangiaceae family remained constant in postlarvae weighing 4 to 10 g. Differences in the taxonomic profile at the family level between culture days were observed through principal component analysis (PCA), where two main clusters were observed: a group of microbiota sampled at 0 D and 20 D and another of samples collected at 40 D, 60 D and 80 D, suggesting that the microbiota tend to be variable during the first postlarval phase but become more constant towards the adult phase. Microbial-mediated functions predicted by PICRUSt showed an overall functional redundancy, suggesting that gut conditions maintain the same microbiota functions regardless of changes in the taxonomic structure. These results also suggest that shrimp are under certain selective pressure favoring microbiota with specific functions according to their requirements.

AB - Even though Litopenaeus vannamei is one of the most important species used in aquaculture, little is known about the functions provided by the intestinal microbiota for host development. This study aims to determine the taxonomic and functional changes in the L. vannamei gut microbiota during postlarval development in a recirculation system under controlled conditions for 80 days. The results revealed that the Vibrionaceae family predominated at the beginning of the experiment, when the postlarvae weighed 0.5 to 1.5 g. The representativeness of the family Rhodobacteraceae increased to the detriment of Vibrionaceae, which showed a constant decrease as the shrimp reached the juvenile and adult stages. Finally, the Intrasporangiaceae family remained constant in postlarvae weighing 4 to 10 g. Differences in the taxonomic profile at the family level between culture days were observed through principal component analysis (PCA), where two main clusters were observed: a group of microbiota sampled at 0 D and 20 D and another of samples collected at 40 D, 60 D and 80 D, suggesting that the microbiota tend to be variable during the first postlarval phase but become more constant towards the adult phase. Microbial-mediated functions predicted by PICRUSt showed an overall functional redundancy, suggesting that gut conditions maintain the same microbiota functions regardless of changes in the taxonomic structure. These results also suggest that shrimp are under certain selective pressure favoring microbiota with specific functions according to their requirements.

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