The bifunctional 6-phosphofructokinase-2/fructose-2,6-bisphosphatase from the shrimp Litopenaeus vannamei: Molecular characterization and down-regulation of expression in response to severe hypoxia

Marissa Flores-Sauceda, Laura Camacho-Jiménez*, Alma B. Peregrino-Uriarte, Lilia Leyva-Carrillo, Aldo Arvizu-Flores, Gloria Yepiz-Plascencia

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Hypoxia is a frequent stressor in marine environments with multiple adverse effects on marine species. The white shrimp Litopenaeus vannamei withstands hypoxic conditions by activating anaerobic metabolism with tissue-specific changes in glycolytic and gluconeogenic enzymes. In animal cells, glycolytic/gluconeogenic fluxes are highly controlled by the levels of fructose-2,6-bisphosphate (F-2,6-P2), a signal metabolite synthesized and degraded by the bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase-2). PFK-2/FBPase-2 has been studied in vertebrates and some invertebrates, but as far as we know, there are no reports on PFK-2/FBPase-2 from crustaceans. In the present work, we obtained cDNA nucleotide sequences corresponding to two mRNAs for PFK-2/FBPase-2 and named them PFKFBP1 (1644 bp) and PFKFBP2 (1566 bp), from the white shrimp L. vannamei. The deduced PFKFBP1 and PFKFBP2 are 547 and 521 amino acids long, respectively. Both proteins share 99.23% of identity, and only differ in 26 additional amino acids present in the kinase domain of the PFKFBP1. The kinase and phosphatase domains are highly conserved in sequence and structure between both isoforms and other proteins from diverse taxa. Total expression of PFKFBP1-2 is tissue-specific, more abundant in gills than in hepatopancreas and undetectable in muscle. Moreover, severe hypoxia (1 mg/L of DO) decreased expression of PFKFBP1-2 in gills while anaerobic glycolysis was induced, as indicated by accumulation of cellular lactate. These results suggest that negative regulation of PFKFBP1-2 at expression level is necessary to set up anaerobic glycolysis in the cells during the response to hypoxia.

Original languageEnglish
Article number111095
JournalComparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology
Volume263
DOIs
StatePublished - Jan 2022

Bibliographical note

Funding Information:
This work was funded by UC-MEXUS CONACyT grant CN-18-108 and CONACyT Ciencia Básica grant A1-S-24557 to GYP.To CONACyT grant A1-S-24557 and UC-MEXUS CONACyT (grant CN-18-108) for funding this work. We thank Dr. Silvia Gómez-Jiménez and the personnel from the Laboratory of Marine Invertebrates Physiology of CIAD for the technical help provided in the hypoxia bioassay. MSF had a SNI-III assistant scholarship from CONACyT. LCJ had a postdoctoral fellowship granted by CONACyT.

Funding Information:
This work was funded by UC-MEXUS CONACyT grant CN-18-108 and CONACyT Ciencia Básica grant A1-S-24557 to GYP.

Funding Information:
To CONACyT grant A1-S-24557 and UC-MEXUS CONACyT (grant CN-18-108 ) for funding this work. We thank Dr. Silvia Gómez-Jiménez and the personnel from the Laboratory of Marine Invertebrates Physiology of CIAD for the technical help provided in the hypoxia bioassay. MSF had a SNI-III assistant scholarship from CONACyT. LCJ had a postdoctoral fellowship granted by CONACyT.

Publisher Copyright:
© 2021 Elsevier Inc.

Keywords

  • Gene expression
  • Glycolysis
  • Hypoxia
  • Lactate
  • PFK-2/FBPase-2
  • Shrimp

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