TY - JOUR
T1 - Uropathogenic E. coli and Hybrid Pathotypes in Mexican Women with Urinary Tract Infections
T2 - A Comprehensive Molecular and Phenotypic Overview
AU - Ballesteros-Monrreal, Manuel G.
AU - Mendez-Pfeiffer, Pablo
AU - Ortíz, Bryan
AU - Bolado-Martínez, Enrique
AU - Álvarez-Ainza, Maritza Lizeth
AU - Enciso-Martínez, Yessica
AU - Arenas-Hernández, Margarita M.P.
AU - Diaz-Murrieta, Betsaida
AU - Barrios-Villa, Edwin
AU - Valencia, Dora
N1 - Publisher Copyright:
© 2024 by the authors.
PY - 2024/6
Y1 - 2024/6
N2 - Uropathogenic Escherichia coli (UPEC) is the main cause of urinary tract infections (UTIs) and carries virulence and resistance factors often found in mobilizable genetic elements, such as plasmids or pathogenicity islands (PAIs). UPEC is part of the extraintestinal pathogenic E. coli (ExPEC), but hybrid strains possessing both diarrheagenic E. coli (DEC) and ExPEC traits, termed “hypervirulent”, present a significant health threat. This study assessed the prevalence of UPEC PAIs, ExPEC sequence types (ST), DEC genes, carbapenemase and extended-spectrum β-lactamase (ESBL) phenotypes, resistance genotypes, and plasmids in 40 clinical isolates of UPEC. Results showed that 72.5% of isolates had PAIs, mainly PAI IV536 (53%). ESBL phenotypes were found in 65% of β-lactam-resistant isolates, with 100% of carbapenem-resistant isolates producing carbapenemase. The predominant ESBL gene was bla CTX-M-2 (60%), and the most common resistance gene in fluoroquinolone and aminoglycoside-resistant isolates was aac(6′)Ib (93%). Plasmids were present in 57% of isolates, and 70% belonged to the ST131 clonal group. Molecular markers for DEC pathotypes were detected in 20 isolates, with 60% classified as hybrid pathotypes. These findings indicate significant pathogenic potential and the presence of hybrid pathotypes in E. coli UTI clinical isolates in the Mexican population.
AB - Uropathogenic Escherichia coli (UPEC) is the main cause of urinary tract infections (UTIs) and carries virulence and resistance factors often found in mobilizable genetic elements, such as plasmids or pathogenicity islands (PAIs). UPEC is part of the extraintestinal pathogenic E. coli (ExPEC), but hybrid strains possessing both diarrheagenic E. coli (DEC) and ExPEC traits, termed “hypervirulent”, present a significant health threat. This study assessed the prevalence of UPEC PAIs, ExPEC sequence types (ST), DEC genes, carbapenemase and extended-spectrum β-lactamase (ESBL) phenotypes, resistance genotypes, and plasmids in 40 clinical isolates of UPEC. Results showed that 72.5% of isolates had PAIs, mainly PAI IV536 (53%). ESBL phenotypes were found in 65% of β-lactam-resistant isolates, with 100% of carbapenem-resistant isolates producing carbapenemase. The predominant ESBL gene was bla CTX-M-2 (60%), and the most common resistance gene in fluoroquinolone and aminoglycoside-resistant isolates was aac(6′)Ib (93%). Plasmids were present in 57% of isolates, and 70% belonged to the ST131 clonal group. Molecular markers for DEC pathotypes were detected in 20 isolates, with 60% classified as hybrid pathotypes. These findings indicate significant pathogenic potential and the presence of hybrid pathotypes in E. coli UTI clinical isolates in the Mexican population.
KW - ERIC
KW - ESBL
KW - hybrid pathotypes
KW - pathogenicity islands
KW - plasmids
KW - urinary tract infection
UR - http://www.scopus.com/inward/record.url?scp=85196901226&partnerID=8YFLogxK
U2 - 10.3390/cimb46060353
DO - 10.3390/cimb46060353
M3 - Artículo
C2 - 38921024
AN - SCOPUS:85196901226
SN - 1467-3037
VL - 46
SP - 5909
EP - 5928
JO - Current issues in molecular biology
JF - Current issues in molecular biology
IS - 6
ER -