Virulence and genotypes of white spot syndrome virus infecting Pacific white shrimp Litopenaeus vannamei in north-western Mexico.

Josefina Ramos-Paredes, Jose Manuel Grijalva-Chon, Cuauhtémoc Ibarra-Gamez

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

© 2017 John Wiley & Sons Ltd White spot syndrome virus (WSSV) has caused substantial global economic impact on aquaculture, and it has been determined that strains can vary in virulence. In this study, the effect of viral load was evaluated by infecting Litopenaeus vannamei with 10-fold serial dilution of tissue infected with strain WSSV Mx-H, and the virulence of four WSSV strains from north-western Mexico was assessed along with their variable number of tandem repeat (VNTR) genotypes in ORF75, ORF94 and ORF125. The LD50of the Mx-H strain was a dilution dose of 10−7.5; the mortality titre was 109.2LD50per gram. In shrimp injected with 102.5to 106.5LD50, no significant virulence differences were evident. Using mortality data, the four WSSV strains grouped into three virulence levels. The Mx-F strain (intermediate virulence) and the Mx-C strain (high virulence) showed more genetic differences than those observed between the Mx-G (low-virulence) and Mx-H (high-virulence) strains, in ORF94 and ORF125. The application of high-viral-load inocula proved useful in determining the different virulence phenotypes of the WSSV strains from the Eastern Pacific.
Original languageEnglish
Pages (from-to)425-435
Number of pages11
JournalJournal of Fish Diseases
Volume40
Issue number10
DOIs
StatePublished - Oct 2017

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