Analysis and Simulation of Batch Affinity Processes Applied to Separation of Biomolecules

The scale-up and optimization of large-scale affinity chromatographic operations is of major industrial importance. In this work, a transport model which includes pore diffusion, external film resistance, and finite kinetic rate, was used to mathematically describe the performance of a batch affinity adsorption system. Experimental data from literature describing the adsorption of β- galactosidase onto anti-β-galactosidase immobilized on porous silica was used as a model system. The mathematical model was solved using the numerical method of lines (MOL) in a MATLAB platform. The use of the transport model is a unique way to predict batch affinity performance as well as to obtain a better understanding of the fundamental mechanisms involved in the bioseparations.