TY - JOUR
T1 - Angiogenic potential of plasma-derived extracellular vesicles from impaired fasting glucose patients
T2 - A pilot study
AU - Castillo-Sanchez, Rocio
AU - Candia-Plata, Maria
AU - Ramirez-Romero, Astrid
AU - Mata-Pineda, Ana
AU - Martinez-Soto, Juan
AU - Lopez-Soto, Luis
AU - Galvan-Moroyoqui, Jose
AU - Palomares, Ramon
AU - Rodriguez-Beas, Cesar
AU - Alvarez-Ramos, Mario
AU - Perez-Salazar, Eduardo
AU - Soto-Guzman, Adriana
N1 - Publisher Copyright:
© 2023 The Author(s).
PY - 2023/4
Y1 - 2023/4
N2 - Background: The goal of this study was to analyze the in vitro effect of plasma-isolated extracellular vesicles (EVs) from patients with impaired fasting glucose (IFG) on cell migration and angiogenic score on human endothelial cells (EA.hy926) cultures. Methods: Plasma samples from five patients with IFG, five with Type 2 diabetes mellitus (T2DM), and five normoglycemic subjects (controls) were used. Plasma-derived EVs were characterized by electron microscopy, dynamic light scattering and Western blotting, vascular endothelial growth factor receptor 2 (VEGFR-2), and endoglin detected in EVs by flow cytometry; wound closure assays and angiogenic score by matrigel assays in EA.hy926 cells were performed. Results: EA.hy926 cell migration induced by plasma-derived EVs from patients with IFG was greater than in control subjects (P = 0.023). EVs from patients with T2DM and IFG induced higher angiogenic scores than EVs from control subjects (P = 0.012 and P = 0.036, respectively). Conclusions: Endoglin and VEGFR-2 levels in EVs from IFG or T2DM patients were not different from those in control subjects. Plasma-derived EVs from patients with IFG and T2DM positively influenced human endothelial cell migration and angiogenic activity in vitro.
AB - Background: The goal of this study was to analyze the in vitro effect of plasma-isolated extracellular vesicles (EVs) from patients with impaired fasting glucose (IFG) on cell migration and angiogenic score on human endothelial cells (EA.hy926) cultures. Methods: Plasma samples from five patients with IFG, five with Type 2 diabetes mellitus (T2DM), and five normoglycemic subjects (controls) were used. Plasma-derived EVs were characterized by electron microscopy, dynamic light scattering and Western blotting, vascular endothelial growth factor receptor 2 (VEGFR-2), and endoglin detected in EVs by flow cytometry; wound closure assays and angiogenic score by matrigel assays in EA.hy926 cells were performed. Results: EA.hy926 cell migration induced by plasma-derived EVs from patients with IFG was greater than in control subjects (P = 0.023). EVs from patients with T2DM and IFG induced higher angiogenic scores than EVs from control subjects (P = 0.012 and P = 0.036, respectively). Conclusions: Endoglin and VEGFR-2 levels in EVs from IFG or T2DM patients were not different from those in control subjects. Plasma-derived EVs from patients with IFG and T2DM positively influenced human endothelial cell migration and angiogenic activity in vitro.
KW - Angiogenesis
KW - extracellular vesicles
KW - impaired fasting glucose
KW - migration
KW - type 2 diabetes
UR - http://www.scopus.com/inward/record.url?scp=85164933290&partnerID=8YFLogxK
U2 - 10.4103/bbrj.bbrj_56_23
DO - 10.4103/bbrj.bbrj_56_23
M3 - Artículo
AN - SCOPUS:85164933290
SN - 2588-9834
VL - 7
SP - 187
EP - 194
JO - Biomedical and Biotechnology Research Journal
JF - Biomedical and Biotechnology Research Journal
IS - 2
ER -