TY - JOUR
T1 - Antimutagenic bis (2-ethylhexyl) phthalate isolated from octopus (paraoctopus vulgaris)
AU - Cruz-Ramírez, Susana Gabriela
AU - López-Saiz, Carmen María
AU - Rosas-Burgos, Ema Carina
AU - Cinco-Moroyoqui, Francisco Javier
AU - Velázquez, Carlos
AU - Hernández, Javier
AU - Burgos-Hernández, Armando
N1 - Publisher Copyright:
© 2021, Sociedade Brasileira de Ciencia e Tecnologia de Alimentos, SBCTA. All rights reserved.
PY - 2021/4/1
Y1 - 2021/4/1
N2 - In the present study, the antimutagenic activity of methanolic (MP) and hexanic (HP) phase from hexane-soluble extract of Paraoctopus vulgaris was evaluated. The HP showed significant concentration-dependent antimutagenic activity (p < 0.05) in the Ames assay against aflatoxin B1 (AFB1 ) using Salmonella typhimurium TA98 and TA100 tester strains. Seven fractions (F1-F7) were obtained from HP by silica gel-column chromatography and the antimutagenic activity of F6 and F7 caused significant reduction (p < 0.05) in the number of AFB1-induced revertants in both strains. However, high antimutagenic activity was exhibited in TA98 strain (85-97% reversion inhibition) and remained at a fraction-concentration of 3 μg/mL. F6 and F7 composition was analyzed by high-performance liquid chromatography/photodiode array, both fractions shared one peak at 9.42 min suggesting presence of the same compound.1H-NMR and13C-NMR analysis results indicate that the bioactive compound is bis (2-ethylhexyl) phthalate. Based on of these results bis (2-ethylhexyl) phthalate exhibit antimutagenic activity, which allows to consider it for further investigation as a potential chemopreventive compound.
AB - In the present study, the antimutagenic activity of methanolic (MP) and hexanic (HP) phase from hexane-soluble extract of Paraoctopus vulgaris was evaluated. The HP showed significant concentration-dependent antimutagenic activity (p < 0.05) in the Ames assay against aflatoxin B1 (AFB1 ) using Salmonella typhimurium TA98 and TA100 tester strains. Seven fractions (F1-F7) were obtained from HP by silica gel-column chromatography and the antimutagenic activity of F6 and F7 caused significant reduction (p < 0.05) in the number of AFB1-induced revertants in both strains. However, high antimutagenic activity was exhibited in TA98 strain (85-97% reversion inhibition) and remained at a fraction-concentration of 3 μg/mL. F6 and F7 composition was analyzed by high-performance liquid chromatography/photodiode array, both fractions shared one peak at 9.42 min suggesting presence of the same compound.1H-NMR and13C-NMR analysis results indicate that the bioactive compound is bis (2-ethylhexyl) phthalate. Based on of these results bis (2-ethylhexyl) phthalate exhibit antimutagenic activity, which allows to consider it for further investigation as a potential chemopreventive compound.
KW - Antimutagenic
KW - Bis (2-ethylhexyl) phthalate
KW - Chemopreventive
KW - Paraoctopus vulgaris
UR - http://www.scopus.com/inward/record.url?scp=85103614898&partnerID=8YFLogxK
U2 - 10.1590/fst.26119
DO - 10.1590/fst.26119
M3 - Artículo
AN - SCOPUS:85103614898
SN - 0101-2061
VL - 41
SP - 314
EP - 320
JO - Food Science and Technology
JF - Food Science and Technology
IS - 2
ER -