TY - JOUR
T1 - Antimycobacterial Precatorin A Flavonoid Displays Antibiofilm Activity against Mycobacterium bovis BCG
AU - Niño-Padilla, Esmeralda Ivonne
AU - Espitia, Clara
AU - Velazquez, Carlos
AU - Alday, Efrain
AU - Silva-Campa, Erika
AU - Burgara-Estrella, Alexel
AU - Enciso-Moreno, José Antonio
AU - Valenzuela, Olivia
AU - Astiazarán-García, Humberto
AU - Garibay-Escobar, Adriana
N1 - Publisher Copyright:
© 2023 The Authors. Published by American Chemical Society.
PY - 2023/10/31
Y1 - 2023/10/31
N2 - The aim of this study was to evaluate the potential antibiofilm activity of Rhynchosia precatoria (R. precatoria) compounds over Mycobacterium bovis BCG (M. bovis BCG) as a model for Mycobacterium tuberculosis (Mtb). We evaluated the antibiofilm activity as the ability to both inhibit biofilm formation and disrupt preformed biofilms (bactericidal) of R. precatoria compounds, which have been previously described as being antimycobacterials against Mtb. M. bovis BCG developed air-liquid interface biofilms with surface attachment ability and drug tolerance. Of the R. precatoria extracts and compounds that were tested, precatorin A (PreA) displayed the best biofilm inhibitory activity, as evaluated by biofilm biomass quantification, viable cell count, and confocal and atomic force microscopy procedures. Furthermore, its combination with isoniazid at subinhibitory concentrations inhibited M. bovis BCG biofilm formation. Nonetheless, neither PreA nor the extract showed bactericidal effects. PreA is the R. precatoria compound responsible for biofilm inhibitory activity against M. bovis BCG.
AB - The aim of this study was to evaluate the potential antibiofilm activity of Rhynchosia precatoria (R. precatoria) compounds over Mycobacterium bovis BCG (M. bovis BCG) as a model for Mycobacterium tuberculosis (Mtb). We evaluated the antibiofilm activity as the ability to both inhibit biofilm formation and disrupt preformed biofilms (bactericidal) of R. precatoria compounds, which have been previously described as being antimycobacterials against Mtb. M. bovis BCG developed air-liquid interface biofilms with surface attachment ability and drug tolerance. Of the R. precatoria extracts and compounds that were tested, precatorin A (PreA) displayed the best biofilm inhibitory activity, as evaluated by biofilm biomass quantification, viable cell count, and confocal and atomic force microscopy procedures. Furthermore, its combination with isoniazid at subinhibitory concentrations inhibited M. bovis BCG biofilm formation. Nonetheless, neither PreA nor the extract showed bactericidal effects. PreA is the R. precatoria compound responsible for biofilm inhibitory activity against M. bovis BCG.
UR - http://www.scopus.com/inward/record.url?scp=85176753731&partnerID=8YFLogxK
U2 - 10.1021/acsomega.3c05703
DO - 10.1021/acsomega.3c05703
M3 - Artículo
C2 - 37929145
AN - SCOPUS:85176753731
SN - 2470-1343
VL - 8
SP - 40665
EP - 40676
JO - ACS Omega
JF - ACS Omega
IS - 43
ER -