TY - JOUR
T1 - Antiproliferative Potential of Olneya tesota PF2 Lectin in Human Acute Monocytic Leukemia Cells
AU - Villegas-Coronado, Diana
AU - Soto-Guzman, Jesús Adriana
AU - Martínez-Soto, Juan Manuel
AU - Teran-Saavedra, Nayelli Guadalupe
AU - Guzman-Partida, Ana Maria
AU - Vazquez-Moreno, Luz
AU - Villalba-Villalba, Ana Gloria
AU - Maldonado, Amir
AU - Lagarda-Diaz, Irlanda
N1 - Publisher Copyright:
© 2023 Wiley-VHCA AG, Zurich, Switzerland.
PY - 2023/7
Y1 - 2023/7
N2 - Acute monocytic leukemia is a type of myeloid leukemia that develops in monocytes. The current clinical therapies for leukemia are unsatisfactory due to their side effects and nonspecificity toward target cells. Some lectins display antitumor activity and may specifically recognize cancer cells by binding to carbohydrate structures on their surface. Therefore, this study evaluated the response of the human monocytic leukemia cell lines THP-1 to the Olneya tesota PF2 lectin. The induction of apoptosis and reactive oxygen species production in PF2-treated cells was evaluated by flow cytometry, and the lectin-THP-1 cell interaction and mitochondrial membrane potential were evaluated by confocal fluorescence microscopy. PF2 genotoxicity was evaluated by DNA fragmentation analysis via gel electrophoresis. The results showed that PF2 binds to THP-1 cells, triggers apoptosis and DNA degradation, changes the mitochondrial membrane potential, and increases reactive oxygen species levels in PF2-treated THP-1 cells. These results suggest the potential use of PF2 for developing alternative anticancer treatments with enhanced specificity.
AB - Acute monocytic leukemia is a type of myeloid leukemia that develops in monocytes. The current clinical therapies for leukemia are unsatisfactory due to their side effects and nonspecificity toward target cells. Some lectins display antitumor activity and may specifically recognize cancer cells by binding to carbohydrate structures on their surface. Therefore, this study evaluated the response of the human monocytic leukemia cell lines THP-1 to the Olneya tesota PF2 lectin. The induction of apoptosis and reactive oxygen species production in PF2-treated cells was evaluated by flow cytometry, and the lectin-THP-1 cell interaction and mitochondrial membrane potential were evaluated by confocal fluorescence microscopy. PF2 genotoxicity was evaluated by DNA fragmentation analysis via gel electrophoresis. The results showed that PF2 binds to THP-1 cells, triggers apoptosis and DNA degradation, changes the mitochondrial membrane potential, and increases reactive oxygen species levels in PF2-treated THP-1 cells. These results suggest the potential use of PF2 for developing alternative anticancer treatments with enhanced specificity.
KW - PF2 lectin
KW - THP-1 cells
KW - acute myeloid leukemia
KW - anticancer
KW - apoptosis
KW - cytotoxic
UR - http://www.scopus.com/inward/record.url?scp=85162896811&partnerID=8YFLogxK
U2 - 10.1002/cbdv.202300051
DO - 10.1002/cbdv.202300051
M3 - Artículo
C2 - 37358490
AN - SCOPUS:85162896811
SN - 1612-1872
VL - 20
JO - Chemistry and Biodiversity
JF - Chemistry and Biodiversity
IS - 7
M1 - e202300051
ER -