TY - JOUR
T1 - Bacteria isolated on TCBS media associated with hatched Artemia cysts of commercial brands
AU - López-Torres, M. A.
AU - Lizárraga-Partida, M. L.
N1 - Funding Information:
This work was supported by the grants CONACYT 225080-5-020 Pñ-1297 and CICESE 623115. L.A. Pérez, M. Cervantes, L. Montoya, J. Peterson, M.L. Unzueta, S. Soto, J. de J. Reyna, C. Mota and F. Montañez kindly sent us close cans of Artemia from their aquacultural hatcheries. We would like to express our special gratitude to the Genesis shrimp farm for letting us work in its hatchery as well as to Guadalupe Vargas-Cárdenas and José Marı́a Dominguez for their technical assistance.
PY - 2001/3/1
Y1 - 2001/3/1
N2 - Viable heterotrophic bacteria (VHB)-cultivated on ZoBell media and TCBS media (TCBSB) were enumerated from cysts that were hatched under laboratory sterile conditions as well as in a commercial hatchery without sterile conditions. Since the focus of this work was to study the TCBSB, 10 to 30 colonies growing on TCBS were isolated randomly from each sample. Results from 26 samples of different commercial brands and lots show that VHB concentration is comprised between 106 to 108 colony forming unites (CFU) per milliliter of Artemia nauplii homogenate. The TCBSB population has show an inverse correlation (R2 = 0.5795, a = 0.05) with cysts age, with values comprised between 101 to 107 CFU/ml. Qualitatively, from 617 isolates, 94% were Gram-positive and only 6% were Gram-negative but oxidase-negative. These basic tests indicate that bacteria of the TCBSB population isolated from Artemia nauplii, do not correspond to Vibrio spp. When cysts of the same brand and same stock where hatched under sterile (laboratory) and nonsterile (commercial hatchery) conditions, Gram-positive bacteria constitute 95% of the bacterial population under sterile manipulation, instant of nonsterile conditions, where Gram-negative bacteria constitute 100% of the bacterial community that was isolated on TCBS medium and that taxonomical studies identified mainly as Vibrio alginolyticus. Those results indicate that Gram-negative heterotrophic bacteria growing on TCBS, normally reported as introduced by Artemia nauplii, are not associated with Artemia cysts but introduced by commercial hatchery operations.
AB - Viable heterotrophic bacteria (VHB)-cultivated on ZoBell media and TCBS media (TCBSB) were enumerated from cysts that were hatched under laboratory sterile conditions as well as in a commercial hatchery without sterile conditions. Since the focus of this work was to study the TCBSB, 10 to 30 colonies growing on TCBS were isolated randomly from each sample. Results from 26 samples of different commercial brands and lots show that VHB concentration is comprised between 106 to 108 colony forming unites (CFU) per milliliter of Artemia nauplii homogenate. The TCBSB population has show an inverse correlation (R2 = 0.5795, a = 0.05) with cysts age, with values comprised between 101 to 107 CFU/ml. Qualitatively, from 617 isolates, 94% were Gram-positive and only 6% were Gram-negative but oxidase-negative. These basic tests indicate that bacteria of the TCBSB population isolated from Artemia nauplii, do not correspond to Vibrio spp. When cysts of the same brand and same stock where hatched under sterile (laboratory) and nonsterile (commercial hatchery) conditions, Gram-positive bacteria constitute 95% of the bacterial population under sterile manipulation, instant of nonsterile conditions, where Gram-negative bacteria constitute 100% of the bacterial community that was isolated on TCBS medium and that taxonomical studies identified mainly as Vibrio alginolyticus. Those results indicate that Gram-negative heterotrophic bacteria growing on TCBS, normally reported as introduced by Artemia nauplii, are not associated with Artemia cysts but introduced by commercial hatchery operations.
KW - Artemia cysts
KW - TCBS
KW - Vibrio alginolyticus
UR - http://www.scopus.com/inward/record.url?scp=0035283748&partnerID=8YFLogxK
U2 - 10.1016/S0044-8486(00)00505-6
DO - 10.1016/S0044-8486(00)00505-6
M3 - Artículo
SN - 0044-8486
VL - 194
SP - 11
EP - 20
JO - Aquaculture
JF - Aquaculture
IS - 1-2
ER -