Batch equilibrium and kinetic studies of plasmid pCI adsorption onto perfusion particles

Y. Pérez-Martínez, R. M. Montesinos-Cisneros, P. Guerrero-Germán, R. Guzman-Zamudio, A. Tejeda-Mansir*

*Autor correspondiente de este trabajo

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

1 Cita (Scopus)

Resumen

The demand for efficient production methods of plasmid DNA (pDNA) has increased vastly in response to rapid advances in the use of pDNA for third-generation vaccines. The process development requires conduct basic studies in batch format to determine the characteristic parameters of the biomolecule-adsorbent system. In this work, the equilibrium and kinetic performance of the plasmid pCI adsorption onto ion-exchange perfusive particles POROS 50 HQ was studied. The isotherm obtained was appropriately described by the Langmuir model, with the parameters q m = 29.15 mg PCI/mL of adsorbent and K d = 0.0145 mg de pCI/mL. These values show a greater capacity and more specific adsorption of pCI onto the anion-exchange (AE) perfusive particles then onto AE adsorptive membranes previously studied. The kinetics of adsorption was described adequately by a lumped resistance model using a kinetic constant of k 1 = 0.0067 mL/mg s.

Idioma originalInglés
Páginas (desde-hasta)196-200
Número de páginas5
PublicaciónJournal of Liquid Chromatography and Related Technologies
Volumen38
N.º2
DOI
EstadoPublicada - 20 ene. 2015

Nota bibliográfica

Publisher Copyright:
© 2015 Taylor & Francis Group, LLC.

Huella

Profundice en los temas de investigación de 'Batch equilibrium and kinetic studies of plasmid pCI adsorption onto perfusion particles'. En conjunto forman una huella única.

Citar esto