Binding of PF2 lectin from Olneya tesota to gut proteins of Zabrotes subfasciatus larvae associated with the insecticidal mechanism

Irlanda Lagarda-Diaz, M. Refugio Robles-Burgeño, Ana M. Guzman-Partida, Dawn Geiser, Joy Winzerling, Luz Vazquez-Moreno

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

4 Citas (Scopus)

Resumen

Zabrotes subfasciatus (Boheman) is the main pest of common beans (Phaselous vulgaris). Wild legume seeds from Olneya tesota contain a lectin, PF2, that shows insecticidal activities against this insect. The binding of PF2 to midgut glycoproteins of 20-day-old larvae was evaluated using PF2 affinity chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the proteins retained on the gel revealed several putative glycoproteins, ranging in mass from 17 to 97 kDa. Subsequent protein digestion and analysis by liquid chromatography-tandem mass spectrometry (LC-MS/ MS) provided amino acid fragments that identified an α-tubulin, cytochrome c oxidase subunit I, an odorant receptor, and a lysozyme from available insect sequence databases. The potential of these proteins to serve as part of the mechanisms involved in the insecticidal activity of PF2 to Z. subfasciatus is discussed. © 2012 American Chemical Society.
Idioma originalInglés estadounidense
Páginas (desde-hasta)2398-2402
Número de páginas5
PublicaciónJournal of Agricultural and Food Chemistry
DOI
EstadoPublicada - 7 mar 2012

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