TY - JOUR
T1 - Comparative Genomics of Three Hybrid-Pathogen Multidrug-Resistant Escherichia coli Strains Isolated from Healthy Donors’ Feces
AU - Ortega-Enríquez, Judith Z.
AU - Martínez-de la Peña, Claudia
AU - Lara-Ochoa, Cristina
AU - Rocha-Gracia, Rosa del Carmen
AU - Barrios-Villa, Edwin
AU - Arenas-Hernández, Margarita M.P.
N1 - Publisher Copyright:
© 2024 by the authors.
PY - 2024/9
Y1 - 2024/9
N2 - The present study shows the genomic characterization of three pathogenic Escherichia coli hybrid strains. All strains were previously characterized as diarrheagenic pathotypes (DEC), obtained from feces. The three sequenced strains have genes that encode adhesins (fimH and iha) and iron uptake systems (iucC and iutA). Antibiotic resistance genes were also found for fluoroquinolone and aminoglycoside families in the three strains. The presence of genomic islands (GIs) in the sequenced study strains presented 100% identity (Ec-25.2) and 99% identity (Ec-36.1) with previously reported Extraintestinal Pathogenic E. coli (ExPEC) strains. The Ec-36.4 strain shared a 99% identity with GI from the Enterotoxigenic E. coli (ETEC) pathotype of the diarrheagenic E. coli strain. Ec-25.2 belongs to ST69 and harbors a FimH27 variant, while Ec-36.1 and Ec-36.4 belong to ST4238 and share a FimH54 variant. Four incompatibility groups associated with conjugative plasmids were identified (IncFIB, IncF11, IncI1, and IncB/O/K/Z), as well as Insertion Sequences and MITEs elements.
AB - The present study shows the genomic characterization of three pathogenic Escherichia coli hybrid strains. All strains were previously characterized as diarrheagenic pathotypes (DEC), obtained from feces. The three sequenced strains have genes that encode adhesins (fimH and iha) and iron uptake systems (iucC and iutA). Antibiotic resistance genes were also found for fluoroquinolone and aminoglycoside families in the three strains. The presence of genomic islands (GIs) in the sequenced study strains presented 100% identity (Ec-25.2) and 99% identity (Ec-36.1) with previously reported Extraintestinal Pathogenic E. coli (ExPEC) strains. The Ec-36.4 strain shared a 99% identity with GI from the Enterotoxigenic E. coli (ETEC) pathotype of the diarrheagenic E. coli strain. Ec-25.2 belongs to ST69 and harbors a FimH27 variant, while Ec-36.1 and Ec-36.4 belong to ST4238 and share a FimH54 variant. Four incompatibility groups associated with conjugative plasmids were identified (IncFIB, IncF11, IncI1, and IncB/O/K/Z), as well as Insertion Sequences and MITEs elements.
KW - Escherichia coli
KW - diarrheagenic pathotype
KW - genome
KW - healthy donors
KW - hybrid pathogens
UR - http://www.scopus.com/inward/record.url?scp=85205325551&partnerID=8YFLogxK
U2 - 10.3390/microbiolres15030095
DO - 10.3390/microbiolres15030095
M3 - Artículo
AN - SCOPUS:85205325551
SN - 2036-7473
VL - 15
SP - 1412
EP - 1424
JO - Microbiology Research
JF - Microbiology Research
IS - 3
ER -