TY - JOUR
T1 - Conductometric uric acid and urea biosensor prepared from electroconductive polyaniline-poly(n-butyl methacrylate) composites
AU - Castillo-Ortega, M. M.
AU - Rodriguez, D. E.
AU - Encinas, J. C.
AU - Plascencia, M.
AU - Méndez-Velarde, F. A.
AU - Olayo, R.
N1 - Funding Information:
This work was supported by the Consejo Nacional de Ciencia y Tecnologia, México (Grant No. 31334-U). The authors thank M. Inoue and M. Barboza for their valuable discussions and F. Brown for his assistance in obtaining SEM images of the composites. One of the authors, J.C. Encinas, thanks Industrias Resistol S.A. for providing the financial support.
PY - 2002/6/20
Y1 - 2002/6/20
N2 - Polyaniline-poly(n-butyl methacrylate) (PAn-PBMA) composite films were obtained by casting. The homogeneous films were prepared with poly(vinyl methyl ether) (PVME) and poly(vinyl ethyl ether) (PVEE) as dispersants, and the electric conductivity, mechanical properties and morphology were studied. The PAn integration to the composite is better when PVEE is used as dispersant, but the conductivity is higher when PVME is used. The composites with PVME were tested as chemical sensors for H2O2 and NH3, showing good performance. These samples were also tested as biosensor for urea and uric acid using urease and uricase enzymes, respectively. The best results were as chemical sensor for H2O2, NH3, and as biosensors for serum urea determination.
AB - Polyaniline-poly(n-butyl methacrylate) (PAn-PBMA) composite films were obtained by casting. The homogeneous films were prepared with poly(vinyl methyl ether) (PVME) and poly(vinyl ethyl ether) (PVEE) as dispersants, and the electric conductivity, mechanical properties and morphology were studied. The PAn integration to the composite is better when PVEE is used as dispersant, but the conductivity is higher when PVME is used. The composites with PVME were tested as chemical sensors for H2O2 and NH3, showing good performance. These samples were also tested as biosensor for urea and uric acid using urease and uricase enzymes, respectively. The best results were as chemical sensor for H2O2, NH3, and as biosensors for serum urea determination.
KW - Composites
KW - Electroconductive polymers
KW - Polyaniline
KW - Urea sensor
KW - Uric acid sensor
UR - http://www.scopus.com/inward/record.url?scp=0037141787&partnerID=8YFLogxK
U2 - 10.1016/S0925-4005(02)00045-X
DO - 10.1016/S0925-4005(02)00045-X
M3 - Artículo
SN - 0925-4005
VL - 85
SP - 19
EP - 25
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
IS - 1-2
ER -