Detection of immunological reaction with the attenuated total reflection (ATR) technique

E. Rodriguez, L.E. Regalado

Producción científica: Capítulo del libro/informe/acta de congresoContribución a la conferenciarevisión exhaustiva

Resumen

The sensitivity of the Attenuated Total Reflection (ATR) technique to surface modifications allows the study of biological or medical phenomena where solutions seggregate to form a layer on the walls of the sample holder. An aluminum thin-film is deposited at the bottom of the container where the reaction takes place and is attached to a prism. During the reaction a layer is adsorbed on the aluminum film. The study of the total reflectivity in this system when the incoming light enters through the prism shows a resonant absorption peak that shifts towards larger angles of incidence when monochromatic light is used. This shift is related to the optical properties and the thickness of the adsorbed layers and may be quantified. We present here the study of Antigen-Antibody reactions with the aid of this technique.

Idioma originalInglés
Título de la publicación alojadaProceedings of SPIE - The International Society for Optical Engineering
Subtítulo de la publicación alojadaOptics for Science and New Technology
Páginas1045-1046
Número de páginas2
EdiciónPART 2
EstadoPublicada - 1996
Evento17th Congress of the International Commission for Optics: Optics for Science and New Technology, ICO 1996 - Taejon, República de Corea
Duración: 19 ago. 199623 ago. 1996

Serie de la publicación

NombreProceedings of SPIE - The International Society for Optical Engineering
NúmeroPART 2
Volumen2778
ISSN (versión impresa)0277-786X
ISSN (versión digital)1996-756X

Conferencia

Conferencia17th Congress of the International Commission for Optics: Optics for Science and New Technology, ICO 1996
País/TerritorioRepública de Corea
CiudadTaejon
Período19/08/9623/08/96

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