TY - JOUR
T1 - European genotype of porcine reproductive and respiratory syndrome (PRRSV) infects monocyte-derived dendritic cells but does not induce Treg cells
AU - Silva-Campa, Erika
AU - Cordoba, Lorena
AU - Fraile, Lorenzo
AU - Flores-Mendoza, Lilian
AU - Montoya, María
AU - Hernández, Jesús
PY - 2010/1/20
Y1 - 2010/1/20
N2 - The aim of this study was to characterize the immune responses of DCs after infection with four different EU strains of PRRSV and whether they show any ability to immunomodulate T cells activation. Our results show that all EU strains can efficiently infect and replicate in DCs. Nevertheless, SLA-II levels remained unaltered in DC infected by all EU PRRSV strains, whereas SLA-I expression was only reduced when strain 2992 was used. IL-10 production was induced by three EU PRRSV strains, being strain 2992 the highest inducer. However, no induction of Treg cells, measured by CD25 and Foxp3 expression on lymphocytes co-cultured with infected DCs, was found. TGF-β induction was not detected in DC infected with any EU strain tested. In conclusion, DCs infected with EU PRRSV strains exhibited an unbalanced ability to stimulate T cell response and was strain dependent. However, Treg cells were not induced, at least in vitro.
AB - The aim of this study was to characterize the immune responses of DCs after infection with four different EU strains of PRRSV and whether they show any ability to immunomodulate T cells activation. Our results show that all EU strains can efficiently infect and replicate in DCs. Nevertheless, SLA-II levels remained unaltered in DC infected by all EU PRRSV strains, whereas SLA-I expression was only reduced when strain 2992 was used. IL-10 production was induced by three EU PRRSV strains, being strain 2992 the highest inducer. However, no induction of Treg cells, measured by CD25 and Foxp3 expression on lymphocytes co-cultured with infected DCs, was found. TGF-β induction was not detected in DC infected with any EU strain tested. In conclusion, DCs infected with EU PRRSV strains exhibited an unbalanced ability to stimulate T cell response and was strain dependent. However, Treg cells were not induced, at least in vitro.
KW - Dendritic cells
KW - Foxp3
KW - IL-10
KW - PRRSV
KW - TGF-β
UR - http://www.scopus.com/inward/record.url?scp=70749155236&partnerID=8YFLogxK
U2 - 10.1016/j.virol.2009.10.024
DO - 10.1016/j.virol.2009.10.024
M3 - Artículo
C2 - 19913865
SN - 0042-6822
VL - 396
SP - 264
EP - 271
JO - Virology
JF - Virology
IS - 2
ER -