@inproceedings{977a7225c10a41169620c209318af930,
title = "Heat in optical tweezers",
abstract = "Laser-induced thermal effects in optically trapped microspheres and single cells have been investigated by Luminescence Thermometry. Thermal spectroscopy has revealed a non-localized temperature distribution around the trap that extends over tens of microns, in agreement with previous theoretical models. Solvent absorption has been identified as the key parameter to determine laser-induced heating, which can be reduced by establishing a continuous fluid flow of the sample. Our experimental results of thermal loading at a variety of wavelengths reveal that an optimum trapping wavelength exists for biological applications close to 820 nm. This has been corroborated by a simultaneous analysis of the spectral dependence of cellular heating and damage in human lymphocytes during optical trapping. Minimum intracellular heating, well below the cytotoxic level (43 °C), has been demonstrated to occur for optical trapping with 820 nm laser radiation, thus avoiding cell damage.",
keywords = "Optical trapping, fluorescence imaging, lymphocytes, microspheres, nanothermometry, quantum dots",
author = "{Del Rosal}, B. and P. Haro-Gonz{\'a}lez and Ramsay, {W. T.} and Maestro, {L. M.} and K. Santacruz-G{\'o}mez and {Iglesias-De La Cruz}, {M. C.} and F. Sanz-Rodr{\'i}guez and Chooi, {J. Y.} and P. Rodr{\'i}guez-Sevilla and D. Choudhury and Kar, {A. K.} and Garc{\'i}a-Sol{\'e}, {J. G.} and L. Patterson and D. Jaque",
year = "2013",
doi = "10.1117/12.2027750",
language = "Ingl{\'e}s",
isbn = "9780819496607",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
booktitle = "Optical Trapping and Optical Micromanipulation X",
note = "Optical Trapping and Optical Micromanipulation X ; Conference date: 25-08-2013 Through 29-08-2013",
}