TY - JOUR
T1 - Inhibition of the antioxidant activity of catalase and superoxide dismutase from Fusarium verticillioides exposed to a Jacquinia macrocarpa antifungal fraction
AU - Valenzuela-Cota, D.F.
AU - Buitimea-Cantúa, G.V.
AU - Plascencia-Jatomea, M.
AU - Cinco-Moroyoqui, F.J.
AU - Martínez-Higuera, A.A.
AU - Rosas-Burgos, E.C.
N1 - Publisher Copyright:
© 2019, © 2019 Taylor & Francis Group, LLC.
PY - 2019
Y1 - 2019
N2 - The aim of this study was to investigate the in vitro effect of an antifungal fraction obtained from Jacquinia macrocarpa plant (JmAF) in the generation of reactive oxygen species (ROS) and the activity of the catalase (CAT) and superoxide dismutase (SOD) enzymes from Fusarium verticillioides, as well as their influence in the viability of the fungus spores. The compounds present in the JmAF were determined by gas chromatography/quadrupole time-of-flight mass spectrometry (GC/QTOF-MS). The effect of the exposition to JmAF on the generation of ROS, as well as in the CAT and SOD activities in F. verticillioides, was determined. The main compounds detected were γ-sitosterol, stephamiersine, betulinol and oleic acid. JmAF showed very high ability in inhibiting the spore viability of F. verticillioides, and their capacity to cause oxidative stress by induction of ROS production. JmAF induced the highest ROS concentration and also inhibited CAT and SOD activities. The results obtained in this study indicate that JmAF is worthy of being considered for the fight against phytopathogenic fungi.
AB - The aim of this study was to investigate the in vitro effect of an antifungal fraction obtained from Jacquinia macrocarpa plant (JmAF) in the generation of reactive oxygen species (ROS) and the activity of the catalase (CAT) and superoxide dismutase (SOD) enzymes from Fusarium verticillioides, as well as their influence in the viability of the fungus spores. The compounds present in the JmAF were determined by gas chromatography/quadrupole time-of-flight mass spectrometry (GC/QTOF-MS). The effect of the exposition to JmAF on the generation of ROS, as well as in the CAT and SOD activities in F. verticillioides, was determined. The main compounds detected were γ-sitosterol, stephamiersine, betulinol and oleic acid. JmAF showed very high ability in inhibiting the spore viability of F. verticillioides, and their capacity to cause oxidative stress by induction of ROS production. JmAF induced the highest ROS concentration and also inhibited CAT and SOD activities. The results obtained in this study indicate that JmAF is worthy of being considered for the fight against phytopathogenic fungi.
KW - CAT
KW - EROS
KW - SOD
KW - spore viability
UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-85066832240&partnerID=MN8TOARS
U2 - 10.1080/03601234.2019.1622978
DO - 10.1080/03601234.2019.1622978
M3 - Artículo
C2 - 31146638
SN - 0360-1234
VL - 54
SP - 647
EP - 654
JO - Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes
JF - Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes
IS - 8
ER -