Isolation, identification and evaluation of a mixed culture of microorganisms for degradation of DDT

Esther Carrillo-Pérez, Arturo Ruiz-Manríquez, Haydée Yeomans-Reina

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

15 Citas (Scopus)

Resumen

A mixed culture capable of degrading DDT was isolated from a mixture of samples taken from contaminated water, soil, and sediments from the Yaqui Valley in Sonora, México. The culture was grown intermitently in a mineral salts medium suplemented with 133 ppm of centrifuged commercial DDT and incubated at 28°C and 150 rpm. Growth was evaluated meassuring protein increase using the Lowry method. The protein containt was correlated to increase of dry mass weight. The culture had a specific growth rate of 0.072/h and a generation time of 9.62 h. The residual DDT was determinated by gas chromatogrphy. The growth was sustained by DDT as a sole source of carbon and was completely consumed in the first 40 h. The metabolites DDD and DDE also present in commercial DDT were completely degraded without showing an increase in concentration throughout the culturing. The microbiological identification of this culture suggested that it was formed mainly by a Gram-negative bacilli. These bacilli were identified as members of the genera Pseudomonas, Neisseria, Moraxella and Acinetobacter.
Idioma originalInglés estadounidense
Páginas (desde-hasta)69-75
Número de páginas7
PublicaciónRevista Internacional de Contaminacion Ambiental
EstadoPublicada - 1 abr 2004

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