TY - JOUR
T1 - Jumbo squid (Dosidicus gigas) mantle collagen
T2 - Extraction, characterization, and potential application in the preparation of chitosan-collagen biofilms
AU - Uriarte-Montoya, Mario Hiram
AU - Arias-Moscoso, Joe Luis
AU - Plascencia-Jatomea, Maribel
AU - Santacruz-Ortega, Hisila
AU - Rouzaud-Sández, Ofelia
AU - Cardenas-Lopez, Jose Luis
AU - Marquez-Rios, Enrique
AU - Ezquerra-Brauer, Josafat Marina
N1 - Funding Information:
We are grateful to Wilfrido Torres Arreola and Armando Burgos Hernández for their technical assistance. This study was supported by CONACYT through Grants 43058/A-1 and 89879 .
PY - 2010/6
Y1 - 2010/6
N2 - Collagen-based biomaterials have been widely used due to its binding capabilities. However the properties and potential use of new collagen sources are still under investigation. Fish by-products are an excellent source of collagen. Thus, acid-soluble collagen (ASC) was extracted, and biochemical and physicochemically characterized from one under-utilized specie, jumbo squid (Dosidicus gigas). In addition, commercial chitosan (95-50%)-ASC (5-50%) blend films were successfully prepared by casting, and characterized by infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC). The molecular masses of the ASC subunits were about 190 kDa, 110 kDa, and 97 kDa, the content of proline and hydroxyproline was 10.9% and 2.8%, respectively. The FT-IR and nuclear magnetic resonance spectra (1H NMR) confirmed collagen peptidic crosslinks, and one endothermic peak was found at 119 °C. The FT-IR spectrum showed that chitosan and ASC remain linked into the films mainly due to hydrogen bonding. The 85:15 (chitosan:ASC) ratio was selected for its thermal and mechanical analyses. The thermograms of this film indicated the presence of two peaks, one at 87-98 °C and the other at 142-182 °C. The chitosan:ASC blend produced a transparent and brittle film, with high percentage of elongation at break, and low tensile strength in comparison to chitosan films. D. gigas mantle might be useful as a new source of plasticizer agent in the preparation of biofilms in composites with chitosan.
AB - Collagen-based biomaterials have been widely used due to its binding capabilities. However the properties and potential use of new collagen sources are still under investigation. Fish by-products are an excellent source of collagen. Thus, acid-soluble collagen (ASC) was extracted, and biochemical and physicochemically characterized from one under-utilized specie, jumbo squid (Dosidicus gigas). In addition, commercial chitosan (95-50%)-ASC (5-50%) blend films were successfully prepared by casting, and characterized by infrared spectroscopy (FT-IR) and differential scanning calorimetry (DSC). The molecular masses of the ASC subunits were about 190 kDa, 110 kDa, and 97 kDa, the content of proline and hydroxyproline was 10.9% and 2.8%, respectively. The FT-IR and nuclear magnetic resonance spectra (1H NMR) confirmed collagen peptidic crosslinks, and one endothermic peak was found at 119 °C. The FT-IR spectrum showed that chitosan and ASC remain linked into the films mainly due to hydrogen bonding. The 85:15 (chitosan:ASC) ratio was selected for its thermal and mechanical analyses. The thermograms of this film indicated the presence of two peaks, one at 87-98 °C and the other at 142-182 °C. The chitosan:ASC blend produced a transparent and brittle film, with high percentage of elongation at break, and low tensile strength in comparison to chitosan films. D. gigas mantle might be useful as a new source of plasticizer agent in the preparation of biofilms in composites with chitosan.
KW - Acid-soluble collagen
KW - Chitosan
KW - Dosidicus gigas
KW - Films
KW - Mantle
UR - http://www.scopus.com/inward/record.url?scp=76749132492&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2010.01.008
DO - 10.1016/j.biortech.2010.01.008
M3 - Artículo
SN - 0960-8524
VL - 101
SP - 4212
EP - 4219
JO - Bioresource Technology
JF - Bioresource Technology
IS - 11
ER -