TY - JOUR
T1 - Mutaciones asociadas con resistencia a rifampicina o isoniazida en aislamientos clínicos de M. tuberculosis de Sonora, México
AU - Bolado-Martínez, Enrique
AU - Pérez-Mendoza, Ansix
AU - Alegría-Morquecho, Francisco Monserrat
AU - Candia-Plata, María del Carmen
AU - Aguayo-Verdugo, María del Rosario
AU - Álvarez-Hernández, Gerardo
PY - 2012
Y1 - 2012
N2 - Objective: To perform the analysis of specific regions of the major genes associated with resistance to isoniazid or rifampin. Materials and methods: Twenty two M. tuberculosis strains, isolated from human samples obtained in Sonora, Mexico. Specific primers for hotspots of the rpoB, katG, inhA genes and the ahpC-oxyR intergenic region were used. The purified PCR products were sequenced. Results: Mutations in the promoter of inhA, the ahpC-oxyR region, and codon 315 of katG and in 451 or 456 codons of rpoB, were identified. Conclusions: Detection of mutations not previously reported requires further genotypic analysis of Mycobacterium tuberculosis isolates in Sonora.
AB - Objective: To perform the analysis of specific regions of the major genes associated with resistance to isoniazid or rifampin. Materials and methods: Twenty two M. tuberculosis strains, isolated from human samples obtained in Sonora, Mexico. Specific primers for hotspots of the rpoB, katG, inhA genes and the ahpC-oxyR intergenic region were used. The purified PCR products were sequenced. Results: Mutations in the promoter of inhA, the ahpC-oxyR region, and codon 315 of katG and in 451 or 456 codons of rpoB, were identified. Conclusions: Detection of mutations not previously reported requires further genotypic analysis of Mycobacterium tuberculosis isolates in Sonora.
KW - Drug resistance
KW - Isoniazid
KW - Mutation
KW - Mycobacterium
KW - Rifampin
UR - http://www.scopus.com/inward/record.url?scp=84864780891&partnerID=8YFLogxK
U2 - 10.1590/S0036-36342012000200013
DO - 10.1590/S0036-36342012000200013
M3 - Artículo
SN - 0036-3634
VL - 54
SP - 167
EP - 170
JO - Salud publica de Mexico
JF - Salud publica de Mexico
IS - 2
ER -