Octopus vulgaris ink extracts exhibit antioxidant, antimutagenic, cytoprotective, antiproliferative, and proapoptotic effects in selected human cancer cell lines

Martin Samuel Hernández-Zazueta, Joel Said García-Romo, Luis Noguera-Artiaga, Iván Luzardo-Ocampo, Ángel Antonio Carbonell-Barrachina, Pablo Taboada-Antelo, Rocio Campos-Vega, Ema Carina Rosas-Burgos, María Guadalupe Burboa-Zazueta, Josafat Marina Ezquerra-Brauer, Juan Manuel Martínez-Soto, Hisila del Carmen Santacruz-Ortega, Armando Burgos-Hernández*

*Autor correspondiente de este trabajo

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

10 Citas (Scopus)

Resumen

Abstract: Cancer is a noncommunicable disease of rising worldwide concern. Marine food products such as Octopus vulgaris ink (OI) could be sources of compounds addressing these concerns. This study aimed to evaluate the antimutagenic, cytoprotective, antiproliferative, proapoptotic, and antioxidant capacity of OI extracts on human cancer cell lines (22Rv1, HeLa, A549). The ARPE-19 cell line was used as a reference human cell line to evaluate the ink's cytotoxicity. The water extract exhibited the highest antimutagenic and cytoprotective effect, but the dichloromethane extract (DM) showed the lowest half lethal concentration against 22Rv1 cells. Structural elucidation of purified DM fractions (F1, F2, F3) identified an unreported compound, N-(2-ozoazepan-3-yl)-pyrrolidine-2-carboxamide (OPC). DM-F2 showed high antiproliferative effect (LC50 = 27.6 µg/mL), reactive species modulation, early-apoptosis induction (42.9%), and nuclei disruption in 22Rv1 cells. In silico analysis predicted high OPC affinity with Cyclin D1 (−6.70 kcal/mol), suggesting its potential impact on cell cycle arrest. These results highlight the antimutagenic, cytoprotective, and antiproliferative potential health benefits derived from underutilized marine food products such as OI. Further investigations at in vitro or in vivo levels are required to elucidate mechanisms and health benefits from OI. Practical Application: O. vulgaris ink is an underutilized marine natural product that could be a source of biological compounds with potential health benefits such as antioxidant activity and cancer prevention.

Idioma originalInglés
Páginas (desde-hasta)587-601
Número de páginas15
PublicaciónJournal of Food Science
Volumen86
N.º2
DOI
EstadoPublicada - 1 feb. 2021

Nota bibliográfica

Funding Information:
Authors would like to thank the Consejo Nacional de Ciencia y Tecnolog?a (CONACyT-Mexico) for the graduate scholarship granted to Martin Samuel Hern?ndez-Zazueta and for the financial support granted to research projects 241133 and 2174. The technical support for this research work, provided by Edgar Sandoval-Petris, Susana Gabriela Cruz-Ram?rez, Alma Carolina G?lvez Iriqui, and Maria del Carmen Candia-Plata, is also well appreciated.

Publisher Copyright:
© 2021 Institute of Food Technologists®

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