TY - JOUR
T1 - Physicochemical characterization of actomyosin–paramyosin from giant squid mantle (Dosidicus gigas)
AU - Tolano-Villaverde, Ivan J.
AU - Ocaño-Higuera, Victor
AU - Ezquerra-Brauer, Josafat
AU - Santos-Sauceda, Irela
AU - Santacruz-Ortega, Hisila
AU - Cárdenas-López, José L.
AU - Rodríguez-Olibarria, Guillermo
AU - Márquez-Ríos, Enrique
N1 - Publisher Copyright:
© 2017 Society of Chemical Industry
PY - 2018/3/30
Y1 - 2018/3/30
N2 - © 2017 Society of Chemical Industry BACKGROUND: The giant squid (Dosidicus gigas) has been proposed as raw material to obtain myofibrillar protein concentrates. However, it has been observed that colloidal systems formed from squid proteins have limited stability. Therefore, the isolation and characterization of the actomyosin–paramyosin isolated (API) complex were performed, because they are the main proteins to which functionality has been attributed. RESULTS: Densitogram analysis revealed 45% of actin, 38% of myosin and 17% of paramyosin. The amino acid profile indicates a higher proportion of acidic amino acids, which gives a higher negative charge; this was supported by the zeta potential. Total sulfhydryl (TSH) content was lower compared with proteins of other aquatic species. CONCLUSION: The higher percentage of actin in relation to myosin, the presence of paramyosin, as well as the low content of sulfhydryl groups, could comprise the main causes of the low technological functional property of proteins from D. gigas mantle. © 2017 Society of Chemical Industry.
AB - © 2017 Society of Chemical Industry BACKGROUND: The giant squid (Dosidicus gigas) has been proposed as raw material to obtain myofibrillar protein concentrates. However, it has been observed that colloidal systems formed from squid proteins have limited stability. Therefore, the isolation and characterization of the actomyosin–paramyosin isolated (API) complex were performed, because they are the main proteins to which functionality has been attributed. RESULTS: Densitogram analysis revealed 45% of actin, 38% of myosin and 17% of paramyosin. The amino acid profile indicates a higher proportion of acidic amino acids, which gives a higher negative charge; this was supported by the zeta potential. Total sulfhydryl (TSH) content was lower compared with proteins of other aquatic species. CONCLUSION: The higher percentage of actin in relation to myosin, the presence of paramyosin, as well as the low content of sulfhydryl groups, could comprise the main causes of the low technological functional property of proteins from D. gigas mantle. © 2017 Society of Chemical Industry.
KW - Dosidicus gigas
KW - actomyosin–paramyosin
KW - amino acids
KW - sulfhydryl
KW - zeta potential
UR - https://www.mendeley.com/catalogue/335a77ce-7148-313a-afca-45f2a322e19a/
U2 - 10.1002/jsfa.8653
DO - 10.1002/jsfa.8653
M3 - Article
C2 - 28862326
SN - 0022-5142
VL - 98
SP - 1787
EP - 1793
JO - Journal of the Science of Food and Agriculture
JF - Journal of the Science of Food and Agriculture
IS - 5
ER -