TY - JOUR
T1 - PLGA nanoparticle preparations by emulsification and nanoprecipitation techniques: effects of formulation parameters
T2 - Effects of formulation parameters
AU - Hernández-Giottonini, Karol Yesenia
AU - Rodríguez-Córdova, Rosalva Josefina
AU - Gutiérrez-Valenzuela, Cindy Alejandra
AU - Peñuñuri-Miranda, Omar
AU - Zavala-Rivera, Paul
AU - Guerrero-Germán, Patricia
AU - Lucero-Acuña, Armando
N1 - Publisher Copyright:
This journal is © The Royal Society of Chemistry.
PY - 2020
Y1 - 2020
N2 - This study presents the influence of the primary formulation parameters on the formation of poly-dl-lactic-co-glycolic nanoparticles by the emulsification-solvent evaporation, and the nanoprecipitation techniques. In the emulsification-solvent evaporation technique, the polymer and tensoactive concentrations, the organic solvent fraction, and the sonication amplitude effects were analyzed. Similarly, in the nanoprecipitation technique the polymer and tensoactive concentrations, the organic solvent fraction and the injection speed were varied. Additionally, the agitation speed during solvent evaporation, the centrifugation speeds and the use of cryoprotectants in the freeze-drying process were analyzed. Nanoparticles were characterized by dynamic light scattering, laser Doppler electrophoresis, and scanning electron microscopy, and the results were evaluated by statistical analysis. Nanoparticle physicochemical characteristics can be adjusted by varying the formulation parameters to obtain specific sizes and stable nanoparticles. Also, by adjusting these parameters, the nanoparticle preparation processes have the potential to be tuned to yield nanoparticles with specific characteristics while maintaining reproducible results.
AB - This study presents the influence of the primary formulation parameters on the formation of poly-dl-lactic-co-glycolic nanoparticles by the emulsification-solvent evaporation, and the nanoprecipitation techniques. In the emulsification-solvent evaporation technique, the polymer and tensoactive concentrations, the organic solvent fraction, and the sonication amplitude effects were analyzed. Similarly, in the nanoprecipitation technique the polymer and tensoactive concentrations, the organic solvent fraction and the injection speed were varied. Additionally, the agitation speed during solvent evaporation, the centrifugation speeds and the use of cryoprotectants in the freeze-drying process were analyzed. Nanoparticles were characterized by dynamic light scattering, laser Doppler electrophoresis, and scanning electron microscopy, and the results were evaluated by statistical analysis. Nanoparticle physicochemical characteristics can be adjusted by varying the formulation parameters to obtain specific sizes and stable nanoparticles. Also, by adjusting these parameters, the nanoparticle preparation processes have the potential to be tuned to yield nanoparticles with specific characteristics while maintaining reproducible results.
UR - http://www.scopus.com/inward/record.url?scp=85079239858&partnerID=8YFLogxK
U2 - 10.1039/c9ra10857b
DO - 10.1039/c9ra10857b
M3 - Artículo
AN - SCOPUS:85079239858
SN - 2046-2069
VL - 10
SP - 4218
EP - 4231
JO - RSC Advances
JF - RSC Advances
IS - 8
ER -