Porcine reproductive and respiratory syndrome virus induces CD4 +CD8 +CD25 +Foxp3 + regulatory T cells (Tregs)

Erika Silva-Campa, Verónica Mata-Haro, Enric Mateu, Jesús Hernández*

*Autor correspondiente de este trabajo

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

70 Citas (Scopus)

Resumen

The aim of this study was to analyze the regulatory T cells (Tregs) induced by the porcine reproductive and respiratory syndrome virus (PRRSV) in pigs. Serum, blood, tonsil, and mediastinal lymph nodes' samples were obtained at different time post-infection (dpi). The frequencies of CD4 +CD8 -CD25 +Foxp3 +, CD4 +CD8 +CD25 +Foxp3 +, or CD4 -CD8 +CD25 +Foxp3 + phenotypes were determined in PBMC and lymph node cells, and cells producing IL-10 or TGF-Β were analyzed. PRRSV increased the number of CD4 +CD8 +CD25 +Foxp3 + cells at 14dpi, whereas CD4 +CD8 -CD25 +Foxp3 + remained constant until 28dpi. Positive correlation exists between viremia and induced regulatory cells. CD4 +CD8 +CD25 +Foxp3 +-induced Treg cells were consistently observed in lymphoid tissues. Analysis of IL-10- and TGF-Β-producing cell demonstrated that in response to PRRSV, CD4 +CD8 -Foxp3 low and CD4 +CD8 +Foxp3 high cells increase moderately the proportion of IL-10 + cells. TGF-Β was only observed in the CD4 +CD8 +Foxp3 high population after PRRSV stimulation. In conclusion, PRRSV infection increases the frequency of Tregs with the phenotype CD4 +CD8 +CD25 +Foxp3 high and produces TGF-Β.

Idioma originalInglés
Páginas (desde-hasta)73-80
Número de páginas8
PublicaciónVirology
Volumen430
N.º1
DOI
EstadoPublicada - 15 ago. 2012
Publicado de forma externa

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