Protective effect of Caffeic Acid Phenethyl Ester (CAPE) against oxidative stress

Ana Laura Carreño, Efrain Alday, Jael Quintero, Lucía Pérez, Dora Valencia, Ramón Robles-Zepeda, Judith Valdez-Ortega, Javier Hernandez, Carlos Velazquez*

*Autor correspondiente de este trabajo

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

17 Citas (Scopus)


The antioxidant properties of several polyphenolics of propolis have been reported, however their protective effect against oxidative stress considering cell integrity is scarce. In this study, we evaluated the cellular antioxidant activity (CAA) of caffeic acid phenetyl ester (CAPE), rutin and galangin (Sonoran propolis constituents) using two murine cell lines derived from different immunological lineages (B-cell lymphoma and macrophages), based on the fluorescence of intracellularly oxidised 2′-7′-dichlorofluorescein (DCF) probe, together with cell morphology analysis and membrane integrity assessment by flow cytometry. CAPE (5 μM) showed the highest CAA (97.9%) on B-cell lymphoma cells against 1 mM H2O2, followed by rutin (25 μM; 30.9%), meanwhile galangin (25 μM) did not show CAA. CAPE exhibited a higher CAA than the antioxidant controls [quercetin (12.5 μM), ascorbic acid (50 μM) and trolox (10 μM)], and additionally it helped to preserve cellular morphology. Similar effects were observed on macrophage cells, indicating that CAPE has a cellular protective effect against ROS. Further studies are needed to investigate the potential health benefits of CAPE.

Idioma originalInglés
Páginas (desde-hasta)178-184
Número de páginas7
PublicaciónJournal of Functional Foods
EstadoPublicada - 1 feb. 2017

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© 2016 Elsevier Ltd


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