TY - JOUR
T1 - Quantitative HPLC analysis of riboflavin and aromatic amino acids in three forms of shrimp hydrolysates
AU - Bueno-Solano, C.
AU - López-Cervantes, J.
AU - Campas-Baypoli, O. N.
AU - Cortez-Rocha, M. O.
AU - Casillas-Hernández, R.
AU - Milaán-Carrillo, J.
AU - Sánchez-Machado, D. I.
PY - 2009/12
Y1 - 2009/12
N2 - This paper presents a high performance liquid chromatography (HPLC) method for the determination of riboflavin and free aromatic amino acids. To determine riboflavin, the method includes an acid hydrolysis (HCl 0.1N) followed by an enzymatic digestion and a protein precipitation with trichloroacetic acid. An analytical column, Chrom SEP SS C18 5m (4.6mm150mm) was used with an isocratic flow using a mobile phase consisting of 5mM ammonium acetate-methanol (72:28, vv-1), at a flow rate of 1.0mL min-1 to 28C. The method showed adequate linearity, repeatability, reproducibility, accuracy, and limits of detection. The method was used to quantify the cited analytes in three different forms of hydrolysate obtained from fermented shrimp remnants.
AB - This paper presents a high performance liquid chromatography (HPLC) method for the determination of riboflavin and free aromatic amino acids. To determine riboflavin, the method includes an acid hydrolysis (HCl 0.1N) followed by an enzymatic digestion and a protein precipitation with trichloroacetic acid. An analytical column, Chrom SEP SS C18 5m (4.6mm150mm) was used with an isocratic flow using a mobile phase consisting of 5mM ammonium acetate-methanol (72:28, vv-1), at a flow rate of 1.0mL min-1 to 28C. The method showed adequate linearity, repeatability, reproducibility, accuracy, and limits of detection. The method was used to quantify the cited analytes in three different forms of hydrolysate obtained from fermented shrimp remnants.
KW - Aromatic amino acids
KW - Fluorescence detection
KW - HPLC
KW - Hydrolysate
KW - Riboflavin
KW - Shrimp remnants
UR - http://www.scopus.com/inward/record.url?scp=71949123744&partnerID=8YFLogxK
U2 - 10.1080/10826070903320616
DO - 10.1080/10826070903320616
M3 - Artículo
SN - 1082-6076
VL - 32
SP - 3009
EP - 3024
JO - Journal of Liquid Chromatography and Related Technologies
JF - Journal of Liquid Chromatography and Related Technologies
IS - 20
ER -