TY - JOUR
T1 - Recovery of phytochemical from three safflower ( Carthamus tinctorius L.) by‐products: Antioxidant properties, protective effect of human erythrocytes and profile by UPLC‐DAD‐MS
AU - Del‐Toro‐Sánchez, Carmen Lizette
AU - Rodríguez‐Félix, Francisco
AU - Cinco‐Moroyoqui, Francisco Javier
AU - Juárez, Josué
AU - Ruiz‐Cruz, Saúl
AU - Wong‐Corral, Francisco Javier
AU - Borboa‐Flores, Jesús
AU - Castro‐Enríquez, Daniela Denisse
AU - Barreras‐Urbina, Carlos Gregorio
AU - Tapia‐Hernández, José Agustín
PY - 2021/9/29
Y1 - 2021/9/29
N2 - Agro-industrial by-products are an important source of phenolic compounds. In this study was to evaluate the antioxidant capacity, the protective effect on human erythrocytes and to identify the phenolic compounds present in of safflower (Carthamus tinctorius L.) by-product by UPLC-DAD-MS. The highest antioxidant activity was for leaf methanolic extract by 2,2-diphenyl-1-picrylhydrazyl (DPPH●) (IC50 9.65 µg/ml) and ABTS●+ (IC50 8.46 µg/ml) methods. The stem of both methanolic and ethanolic extracts presented the best protection effect on human erythrocytes (91.86 ± 0.72% and 87.55 ± 0.61%, respectively). Total phenols (6.44 ± 0.18 mg gallic acid equivalent/g) and flavonoids (11.22 ± 0.023 mg quercetin equivalent /g) were higher in leaf followed by mixture (stem + leaf) and stem in methanolic and ethanolic extracts. The major compounds identified by UPLC-DAD-MS were luteolin 7-O-β-D-glucoside, quercetin-3-galactoside, and hydroxysafflor yellow A. Therefore, safflower by-products are sources of obtaining phenolic compounds with application in the food or pharmaceutical areas.
AB - Agro-industrial by-products are an important source of phenolic compounds. In this study was to evaluate the antioxidant capacity, the protective effect on human erythrocytes and to identify the phenolic compounds present in of safflower (Carthamus tinctorius L.) by-product by UPLC-DAD-MS. The highest antioxidant activity was for leaf methanolic extract by 2,2-diphenyl-1-picrylhydrazyl (DPPH●) (IC50 9.65 µg/ml) and ABTS●+ (IC50 8.46 µg/ml) methods. The stem of both methanolic and ethanolic extracts presented the best protection effect on human erythrocytes (91.86 ± 0.72% and 87.55 ± 0.61%, respectively). Total phenols (6.44 ± 0.18 mg gallic acid equivalent/g) and flavonoids (11.22 ± 0.023 mg quercetin equivalent /g) were higher in leaf followed by mixture (stem + leaf) and stem in methanolic and ethanolic extracts. The major compounds identified by UPLC-DAD-MS were luteolin 7-O-β-D-glucoside, quercetin-3-galactoside, and hydroxysafflor yellow A. Therefore, safflower by-products are sources of obtaining phenolic compounds with application in the food or pharmaceutical areas.
UR - https://doi.org/10.1111/jfpp.15765
U2 - 10.1111/jfpp.15765
DO - 10.1111/jfpp.15765
M3 - Artículo
SN - 0145-8892
VL - 45
JO - Journal of Food Processing and Preservation
JF - Journal of Food Processing and Preservation
IS - 9
ER -