TY - JOUR
T1 - Silencing of HIF-1 in WSSV-infected white shrimp
T2 - Effect on viral load and antioxidant enzymes
AU - Miranda-Cruz, Melissa M
AU - Poom-Llamas, Jennifer J
AU - Godoy-Lugo, José A
AU - Ortiz, Rudy M
AU - Gómez-Jiménez, Silvia
AU - Rosas-Rodríguez, Jesús A
AU - Morán-Palacio, Edgar F
AU - Soñanez-Organis, José G
N1 - Copyright © 2018 Elsevier Inc. All rights reserved.
PY - 2018/11
Y1 - 2018/11
N2 - Hypoxia inducible factor-1 (HIF-1) is a transcriptional factor that induces genes involved in glucose metabolism. HIF-1 is formed by a regulatory α-subunit (HIF-1α) and a constitutive β-subunit (HIF-1β). The white spot syndrome virus (WSSV) induces a shift in glucose metabolism and oxidative stress. HIF-1α is associated with the induction of metabolic changes in tissues of WSSV-infected shrimp. However, the contributions of HIF-1 to viral load and antioxidant responses in WSSV-infected shrimp have been not examined. In this study, the effect of HIF-1 silencing on viral load and the expression and activity of antioxidant enzymes (superoxide dismutase-SOD, glutathione S-transferase-GST, and catalase) along with oxidative damage (lipid peroxidation and protein carbonyl) in tissues of white shrimp infected with the WSSV were studied. The viral load increased in hepatopancreas and muscle after WSSV infection, and the accumulative mortality was of 100% at 72 h post-infection. The expression and activity of SOD, catalase, and GST decreased in each tissue evaluated after WSSV infection. Protein carbonyl concentrations increased in each tissue after WSSV infection, while lipid peroxidation increased in hepatopancreas, but not in muscle. Silencing of HIF-1α decreased the WSSV viral load in hepatopancreas and muscle of infected shrimp along with shrimp mortality. Silencing of HIF-1α ameliorated the antioxidant response in a tissue-specific manner, which translated to a decrease in oxidative damage. These results suggest that HIF-1 is essential for restoring the antioxidant response, which counters the oxidative injury associated with WSSV infection.
AB - Hypoxia inducible factor-1 (HIF-1) is a transcriptional factor that induces genes involved in glucose metabolism. HIF-1 is formed by a regulatory α-subunit (HIF-1α) and a constitutive β-subunit (HIF-1β). The white spot syndrome virus (WSSV) induces a shift in glucose metabolism and oxidative stress. HIF-1α is associated with the induction of metabolic changes in tissues of WSSV-infected shrimp. However, the contributions of HIF-1 to viral load and antioxidant responses in WSSV-infected shrimp have been not examined. In this study, the effect of HIF-1 silencing on viral load and the expression and activity of antioxidant enzymes (superoxide dismutase-SOD, glutathione S-transferase-GST, and catalase) along with oxidative damage (lipid peroxidation and protein carbonyl) in tissues of white shrimp infected with the WSSV were studied. The viral load increased in hepatopancreas and muscle after WSSV infection, and the accumulative mortality was of 100% at 72 h post-infection. The expression and activity of SOD, catalase, and GST decreased in each tissue evaluated after WSSV infection. Protein carbonyl concentrations increased in each tissue after WSSV infection, while lipid peroxidation increased in hepatopancreas, but not in muscle. Silencing of HIF-1α decreased the WSSV viral load in hepatopancreas and muscle of infected shrimp along with shrimp mortality. Silencing of HIF-1α ameliorated the antioxidant response in a tissue-specific manner, which translated to a decrease in oxidative damage. These results suggest that HIF-1 is essential for restoring the antioxidant response, which counters the oxidative injury associated with WSSV infection.
KW - Animals
KW - Aquaculture
KW - DNA, Viral/isolation & purification
KW - Gene Expression Regulation, Developmental
KW - Gene Silencing
KW - Hepatopancreas/growth & development
KW - Hypoxia-Inducible Factor 1, alpha Subunit/antagonists & inhibitors
KW - Injections, Intramuscular
KW - Lipid Peroxidation
KW - Mexico
KW - Muscles/metabolism
KW - Organ Specificity
KW - Oxidative Stress
KW - Oxidoreductases/genetics
KW - Penaeidae/growth & development
KW - Protein Carbonylation
KW - RNA Interference
KW - RNA, Double-Stranded/administration & dosage
KW - Viral Load
KW - White spot syndrome virus 1/isolation & purification
U2 - 10.1016/j.cbpc.2018.07.004
DO - 10.1016/j.cbpc.2018.07.004
M3 - Artículo
C2 - 30041062
SN - 1532-0456
VL - 213
SP - 19
EP - 26
JO - Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
JF - Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
ER -