TY - JOUR
T1 - Taxonomic and functional changes in the microbiota of the white shrimp (Litopenaeus vannamei) associated with postlarval ontogenetic development
AU - Garibay-Valdez, Estefanía
AU - Martínez-Porchas, Marcel
AU - Calderon, Kadiya
AU - Vargas-Albores, Francisco
AU - Gollas-Galván, Teresa
AU - Martínez Córdova, Luis Rafael
N1 - Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2020
Y1 - 2020
N2 - Even though Litopenaeus vannamei is one of the most important species used in aquaculture, little is known about the functions provided by the intestinal microbiota for host development. This study aims to determine the taxonomic and functional changes in the L. vannamei gut microbiota during postlarval development in a recirculation system under controlled conditions for 80 days. The results revealed that the Vibrionaceae family predominated at the beginning of the experiment, when the postlarvae weighed 0.5 to 1.5 g. The representativeness of the family Rhodobacteraceae increased to the detriment of Vibrionaceae, which showed a constant decrease as the shrimp reached the juvenile and adult stages. Finally, the Intrasporangiaceae family remained constant in postlarvae weighing 4 to 10 g. Differences in the taxonomic profile at the family level between culture days were observed through principal component analysis (PCA), where two main clusters were observed: a group of microbiota sampled at 0 D and 20 D and another of samples collected at 40 D, 60 D and 80 D, suggesting that the microbiota tend to be variable during the first postlarval phase but become more constant towards the adult phase. Microbial-mediated functions predicted by PICRUSt showed an overall functional redundancy, suggesting that gut conditions maintain the same microbiota functions regardless of changes in the taxonomic structure. These results also suggest that shrimp are under certain selective pressure favoring microbiota with specific functions according to their requirements.
AB - Even though Litopenaeus vannamei is one of the most important species used in aquaculture, little is known about the functions provided by the intestinal microbiota for host development. This study aims to determine the taxonomic and functional changes in the L. vannamei gut microbiota during postlarval development in a recirculation system under controlled conditions for 80 days. The results revealed that the Vibrionaceae family predominated at the beginning of the experiment, when the postlarvae weighed 0.5 to 1.5 g. The representativeness of the family Rhodobacteraceae increased to the detriment of Vibrionaceae, which showed a constant decrease as the shrimp reached the juvenile and adult stages. Finally, the Intrasporangiaceae family remained constant in postlarvae weighing 4 to 10 g. Differences in the taxonomic profile at the family level between culture days were observed through principal component analysis (PCA), where two main clusters were observed: a group of microbiota sampled at 0 D and 20 D and another of samples collected at 40 D, 60 D and 80 D, suggesting that the microbiota tend to be variable during the first postlarval phase but become more constant towards the adult phase. Microbial-mediated functions predicted by PICRUSt showed an overall functional redundancy, suggesting that gut conditions maintain the same microbiota functions regardless of changes in the taxonomic structure. These results also suggest that shrimp are under certain selective pressure favoring microbiota with specific functions according to their requirements.
KW - Functional profile
KW - Gut microbiota
KW - Litopenaeus vannamei
KW - Shrimp microbiota
KW - Taxonomic profile
UR - http://www.scopus.com/inward/record.url?scp=85076250926&partnerID=8YFLogxK
U2 - 10.1016/j.aquaculture.2019.734842
DO - 10.1016/j.aquaculture.2019.734842
M3 - Article
SN - 0044-8486
VL - 518
SP - 734842
JO - Aquaculture
JF - Aquaculture
M1 - 734842
ER -