TY - JOUR
T1 - The Dichloromethane Fraction of Croton sonorae, A Plant Used in Sonoran Traditional Medicine, Affect Entamoeba histolytica Erythrophagocytosis and Gene Expression
AU - Villegas-Gómez, Isaac
AU - Silva-Olivares, Angélica
AU - Robles-Zepeda, Ramón Enrique
AU - Gálvez-Ruiz, Juan Carlos
AU - Shibayama, Mineko
AU - Valenzuela, Olivia
N1 - Funding Information:
The authors wish to dedicate this manuscript to the memory of our friend and colleague Mineko Shibayama, whose life has been sadly claimed by the currently ongoing COVID?19 pandemic.
Publisher Copyright:
© Copyright © 2021 Villegas-Gómez, Silva-Olivares, Robles-Zepeda, Gálvez-Ruiz, Shibayama and Valenzuela.
PY - 2021/7/23
Y1 - 2021/7/23
N2 - Intestinal parasites are a global problem, mainly in developing countries. Obtaining information about plants and compounds that can combat gastrointestinal disorders and gastrointestinal symptoms is a fundamental first step in designing new treatment strategies. In this study, we analyzed the antiamoebic activity of the aerial part of Croton sonorae. The dichloromethane fraction of C. sonorae (CsDCMfx) contained flavonoids, terpenes, alkaloids, and glycosides. The ultrastructural morphology of the amoebae treated for 72 h with CsDCMfx was completely abnormal. CsDCMfx reduced erythrophagocytosis of trophozoites and the expression of genes involved in erythrocyte adhesion (gal/galnac lectin) and actin cytoskeleton rearrangement in the phagocytosis pathway (rho1 gtpase and formin1). Interestingly, CsDCMfx decreased the expression of genes involved in Entamoeba histolytica trophozoite pathogenesis, such as cysteine proteases (cp1, cp4, and cp5), sod, pfor, and enolase. These results showed that C. sonorae is a potential source of antiamoebic compounds.
AB - Intestinal parasites are a global problem, mainly in developing countries. Obtaining information about plants and compounds that can combat gastrointestinal disorders and gastrointestinal symptoms is a fundamental first step in designing new treatment strategies. In this study, we analyzed the antiamoebic activity of the aerial part of Croton sonorae. The dichloromethane fraction of C. sonorae (CsDCMfx) contained flavonoids, terpenes, alkaloids, and glycosides. The ultrastructural morphology of the amoebae treated for 72 h with CsDCMfx was completely abnormal. CsDCMfx reduced erythrophagocytosis of trophozoites and the expression of genes involved in erythrocyte adhesion (gal/galnac lectin) and actin cytoskeleton rearrangement in the phagocytosis pathway (rho1 gtpase and formin1). Interestingly, CsDCMfx decreased the expression of genes involved in Entamoeba histolytica trophozoite pathogenesis, such as cysteine proteases (cp1, cp4, and cp5), sod, pfor, and enolase. These results showed that C. sonorae is a potential source of antiamoebic compounds.
KW - amoebicidal effect
KW - Croton sonorae
KW - Entamoeba histolytica
KW - erythrophagocytosis
KW - gene expression
KW - ultrastructural changes
UR - http://www.scopus.com/inward/record.url?scp=85112630457&partnerID=8YFLogxK
U2 - 10.3389/fcimb.2021.693449
DO - 10.3389/fcimb.2021.693449
M3 - Artículo
C2 - 34368014
AN - SCOPUS:85112630457
SN - 2235-2988
VL - 11
JO - Frontiers in Cellular and Infection Microbiology
JF - Frontiers in Cellular and Infection Microbiology
M1 - 693449
ER -