The effect of inoculation time and inoculum concentration on the productive response of Tetraselmis chuii (Butcher, 1958) mass cultured in F/2 and 2-F media.

Diverse protocols have been used in the shrimp industry to produce microalgae. In this work, we evaluated the effect of inoculation time (0600 and 1200) and initial microalgae density (0.4 and 0.8 × 10⁵cells mL^-1) in static outdoor cultures of Tetraselmis chuii, using F/2 and 2-F media. A significant effect on cell density (> 6.7 × 10⁵ cells mL-1) was found after treatments with a high initial density and in the F/2 medium. The higher productive responses were observed in treatments inoculated at 0600. The better productive response was found in treatments inoculated at 0600 with 0.8 × 10⁵ cells·mL^-1 in the F/2 medium.