Virulence and genotypes of white spot syndrome virus infecting Pacific white shrimp Litopenaeus vannamei in north-western Mexico.

Josefina Ramos-Paredes, Jose Manuel Grijalva-Chon, Cuauhtémoc Ibarra-Gamez

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

8 Citas (Scopus)

Resumen

White spot syndrome virus (WSSV) has caused substantial global economic impact on aquaculture, and it has been determined that strains can vary in virulence. In this study, the effect of viral load was evaluated by infecting Litopenaeus vannamei with 10-fold serial dilution of tissue infected with strain WSSV Mx-H, and the virulence of four WSSV strains from north-western Mexico was assessed along with their variable number of tandem repeat (VNTR) genotypes in ORF75, ORF94 and ORF125. The LD50 of the Mx-H strain was a dilution dose of 10−7.5; the mortality titre was 109.2 LD50 per gram. In shrimp injected with 102.5 to 106.5 LD50, no significant virulence differences were evident. Using mortality data, the four WSSV strains grouped into three virulence levels. The Mx-F strain (intermediate virulence) and the Mx-C strain (high virulence) showed more genetic differences than those observed between the Mx-G (low-virulence) and Mx-H (high-virulence) strains, in ORF94 and ORF125. The application of high-viral-load inocula proved useful in determining the different virulence phenotypes of the WSSV strains from the Eastern Pacific.

Idioma originalInglés
Páginas (desde-hasta)425-435
Número de páginas11
PublicaciónJournal of Fish Diseases
Volumen40
N.º10
DOI
EstadoPublicada - oct. 2017

Nota bibliográfica

Publisher Copyright:
© 2017 John Wiley & Sons Ltd

Huella

Profundice en los temas de investigación de 'Virulence and genotypes of white spot syndrome virus infecting Pacific white shrimp Litopenaeus vannamei in north-western Mexico.'. En conjunto forman una huella única.

Citar esto